Name: Anti-HDAC1 antibody [EPR460(2)]
Brand: RabMAb
SKU: ab109411
Rating: 4 (6 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Add compatible products

Primary

Secondary

Protein

Unfortunately, one or more of the products below are unavailable in your country/region.

Sorry, we can't display this right now.
Please contact us to place your order, or try again later.

Primary - Rabbit Anti-HDAC1 antibody [EPR460(2)] (ab109411)
ICC, ICC/IF, WB, Flow Cyt, IHC-P

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

Protein - Recombinant human HDAC1 protein (ab101661)
Functional Studies, WB

View more associated products

Product name

Anti-HDAC1 antibody [EPR460(2)]
See all HDAC1 primary antibodies
Description

Rabbit monoclonal [EPR460(2)] to HDAC1
Host species

Rabbit
Tested applications

Suitable for: ICC, ICC/IF, WB, Flow Cyt, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human HDAC1 aa 400-500 (C terminal). The exact sequence is proprietary.
(Peptide available as ab174565)
Positive control
- Jurkat, HeLa, K562, and MCF7 cell lysates; Human colon tissue; Human tonsil tissue
General notes

A trial size is available to purchase for this antibody.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR460(2)
Isotype

IgG
Research areas

Alternative Versions
Assay kits
- Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa nuclear extract lysate (ab14655)
Recombinant Protein
- Recombinant human HDAC1 protein (ab101661)

Our Abpromise guarantee covers the use of ab109411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC		1/250 - 1/500.
ICC/IF		1/400. Use with paraformaldehyde fixed cells.
WB		1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
Flow Cyt		1/50.
IHC-P		1/50 - 1/100. Antigen retrieval is recommended.

Application notes

Is unsuitable for IP.

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
Tissue specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
- Unigene: 88556 Human
- Unigene: 202504 Mouse
- Unigene: 391033 Mouse
- Unigene: 1863 Rat
see all
Alternative names
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
- HDAC 1 antibody
- HDAC1 antibody
- HDAC1_HUMAN antibody
- Histone deacetylase 1 antibody
- Reduced potassium dependency yeast homolog like 1 antibody
- RPD3 antibody
- RPD3L1 antibody
see all

Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109411 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab109411 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging
Immunohistochemistry paraffin embedded sections - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Overlay histogram showing HeLa cells stained with ab109411 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab109441, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 0.1ug/1x10⁶ cells) used under the same conditions.

Acquisition of >5,000 events were collected using an 50 mW Blue laser (488nm) with 530/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

ab109411 (1/400) staining HDAC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
See Abreview
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

This western blot image is a comparison between ab109411 and a competitor's top cited rabbit polyclonal antibody.
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

Lane 1 : Mouse brain lysate, 20ug
Lane 2 : Mouse heart lysate, 20ug
Lane 3 : Mouse Kidney lysate, 20ug
Lane 4 : Mouse spleen lysate,20ug

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce). at 1/1000 dilution

Predicted band size: 55 kDa
Observed band size: 62 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and Diluting buffer: 5% NFDM /TBST
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 55 kDa
Immunohistochemistry paraffin embedded sections - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Xiong W et al. MiR-761 inhibits colorectal cancer cell proliferation and invasion through targeting HDAC1. Pharmazie 74:111-114 (2019). Read more (PubMed: 30782261) »
Bartosz P et al. Benzophenone-3 passes through the blood-brain barrier, increases the level of extracellular glutamate and induces apoptotic processes in the hippocampus and frontal cortex of rats. Toxicol Sci N/A:N/A (2019). Read more (PubMed: 31368502) »

See all 15 Publications for this product

Publishing research using ab109411? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review

1-6 of 6 Abreviews

Application

Western blot

Sample

Human Cell lysate - whole cell (Brain)

Gel Running Conditions

Reduced Denaturing (4-20%)

Loading amount

12 µg

Treatment

Aliquoted and heated from 34C to 69C to determine melting temperature of HDAC1. Lysate centrifuged at 18,000 x g to remove aggregates after melting. Supernatent collected, 3X SDS PAGE loading dye added, and heated sampled were heated to 95C for 10 min. Note, input sample was not heated before centrifugation, but was heated to 95C after loading dye added.

Specification

Brain

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 17 2015

Application

Western blot

Loading amount

15 µg

Gel Running Conditions

Reduced Denaturing (4-12% Tris-bis glycine gel)

Sample

Mouse Tissue lysate - other (mouse brain tissue)

Specification

mouse brain tissue

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Arjang Salehi

Verified customer

Submitted Mar 26 2015

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Cell lysate - nuclear (B cells)

Specification

B cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 16 2015

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Cell lysate - whole cell (heart)

Specification

heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 13 2015

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C

Sample

Mouse Cell (Neural cell)

Specification

Neural cell

Permeabilization

Yes - 0.3% TritonX-100 in PBS for 30 minutes

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 13 2014

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Specification

HeLa

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.5% Triton-X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted May 31 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC1 antibody [EPR460(2)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC1 antibody [EPR460(2)]

Post-translational
modifications