

Recombinant Anti-HDAC1 antibody [EPR460(2)] (ab109411)
- Datasheet
- References (15)
- Protocols
Overview
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Product name
Anti-HDAC1 antibody [EPR460(2)]
See all HDAC1 primary antibodies -
Description
Rabbit monoclonal [EPR460(2)] to HDAC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, ICC/IF, WB, Flow Cyt, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human HDAC1 aa 400-500 (C terminal). The exact sequence is proprietary.
(Peptide available asab174565) -
Positive control
- Jurkat, HeLa, K562, and MCF7 cell lysates; Human colon tissue; Human tonsil tissue
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General notes
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR460(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC | 1/250 - 1/500. | |
ICC/IF | 1/400. Use with paraformaldehyde fixed cells. |
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WB | 1/1000 - 1/10000. Predicted molecular weight: 55 kDa. | |
Flow Cyt | 1/50. | |
IHC-P | 1/50 - 1/100. Antigen retrieval is recommended. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
Tissue specificity
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
- Unigene: 88556 Human
see all -
Alternative names
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109411 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab109411 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging -
Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Overlay histogram showing HeLa cells stained with ab109411 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab109441, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 0.1ug/1x106 cells) used under the same conditions.
Acquisition of >5,000 events were collected using an 50 mW Blue laser (488nm) with 530/30 bandpass filter.
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Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab109411 (1/400) staining HDAC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.This western blot image is a comparison between ab109411 and a competitor's top cited rabbit polyclonal antibody.
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All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution
Lane 1 : Mouse brain lysate, 20ug
Lane 2 : Mouse heart lysate, 20ug
Lane 3 : Mouse Kidney lysate, 20ug
Lane 4 : Mouse spleen lysate,20ug
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce). at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and Diluting buffer: 5% NFDM /TBST
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All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa -
Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Xiong W et al. MiR-761 inhibits colorectal cancer cell proliferation and invasion through targeting HDAC1. Pharmazie 74:111-114 (2019). Read more (PubMed: 30782261) »
- Bartosz P et al. Benzophenone-3 passes through the blood-brain barrier, increases the level of extracellular glutamate and induces apoptotic processes in the hippocampus and frontal cortex of rats. Toxicol Sci N/A:N/A (2019). Read more (PubMed: 31368502) »