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  1. Link

    hdac1-antibody-epr4602-ab109411.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-HDAC1 antibody [EPR460(2)] (ab109411)

  • Datasheet
Reviews (6) Submit a question References (15)

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  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunohistochemistry paraffin embedded sections - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Flow Cytometry - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunohistochemistry paraffin embedded sections - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

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  • Datasheet
  • References (15)
  • Protocols

Overview

  • Product name

    Anti-HDAC1 antibody [EPR460(2)]
    See all HDAC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR460(2)] to HDAC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HDAC1 aa 400-500 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab174565)

  • Positive control

    • Jurkat, HeLa, K562, and MCF7 cell lysates; Human colon tissue; Human tonsil tissue
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR460(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • HDAC1

Associated products

  • Alternative Versions

    • Anti-HDAC1 antibody [EPR460(2)] (Alexa Fluor® 488) (ab192469)
    • Anti-HDAC1 antibody [EPR460(2)] (Alexa Fluor® 647) (ab192470)
    • Anti-HDAC1 antibody [EPR460(2)] - BSA and Azide free (ab213701)
  • Assay kits

    • Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
  • Recombinant Protein

    • Recombinant human HDAC1 protein (ab101661)

Applications

Our Abpromise guarantee covers the use of ab109411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/250 - 1/500.
ICC/IF 1/400.

Use with paraformaldehyde fixed cells.

WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
Flow Cyt 1/50.
IHC-P 1/50 - 1/100.

Antigen retrieval is recommended.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
    • Tissue specificity

      Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      modifications

      Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
      Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q13547 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3065 Human
      • Entrez Gene: 433759 Mouse
      • Entrez Gene: 297893 Rat
      • Omim: 601241 Human
      • SwissProt: Q13547 Human
      • SwissProt: O09106 Mouse
      • SwissProt: Q4QQW4 Rat
      • Unigene: 88556 Human
      • Unigene: 202504 Mouse
      • Unigene: 391033 Mouse
      • Unigene: 1863 Rat
      see all
    • Alternative names

      • DKFZp686H12203 antibody
      • GON 10 antibody
      • HD1 antibody
      • HDAC 1 antibody
      • HDAC1 antibody
      • HDAC1_HUMAN antibody
      • Histone deacetylase 1 antibody
      • Reduced potassium dependency yeast homolog like 1 antibody
      • RPD3 antibody
      • RPD3L1 antibody
      see all

    Images

    • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human breast carcinoma lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109411 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab109411 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging

    • Immunohistochemistry paraffin embedded sections - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      Immunohistochemistry paraffin embedded sections - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

      Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      Flow Cytometry - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

      Overlay histogram showing HeLa cells stained with ab109411 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab109441, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 0.1ug/1x106 cells) used under the same conditions.

      Acquisition of >5,000 events were collected using an 50 mW Blue laser (488nm) with 530/30 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
      ab109411 (1/400) staining HDAC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

      This western blot image is a comparison between ab109411 and a competitor's top cited rabbit polyclonal antibody.

    • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

      Lane 1 : Mouse brain lysate, 20ug
      Lane 2 : Mouse heart lysate, 20ug
      Lane 3 : Mouse Kidney lysate, 20ug
      Lane 4 : Mouse spleen lysate,20ug

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce). at 1/1000 dilution

      Predicted band size: 55 kDa
      Observed band size: 62 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and Diluting buffer: 5% NFDM /TBST

    • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa

    • Immunohistochemistry paraffin embedded sections - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
      Immunohistochemistry paraffin embedded sections - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

      Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Xiong W  et al. MiR-761 inhibits colorectal cancer cell proliferation and invasion through targeting HDAC1. Pharmazie 74:111-114 (2019). Read more (PubMed: 30782261) »
    • Bartosz P  et al. Benzophenone-3 passes through the blood-brain barrier, increases the level of extracellular glutamate and induces apoptotic processes in the hippocampus and frontal cortex of rats. Toxicol Sci N/A:N/A (2019). Read more (PubMed: 31368502) »
    See all 15 Publications for this product

    Publishing research using ab109411? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-6 of 6 Abreviews

    Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    12 µg
    Treatment
    Aliquoted and heated from 34C to 69C to determine melting temperature of HDAC1. Lysate centrifuged at 18,000 x g to remove aggregates after melting. Supernatent collected, 3X SDS PAGE loading dye added, and heated sampled were heated to 95C for 10 min. Note, input sample was not heated before centrifugation, but was heated to 95C after loading dye added.
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 17 2015

    Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Tris-bis glycine gel)
    Sample
    Mouse Tissue lysate - other (mouse brain tissue)
    Specification
    mouse brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Arjang Salehi

    Verified customer

    Submitted Mar 26 2015

    Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - nuclear (B cells)
    Specification
    B cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2015

    Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (heart)
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 13 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC1 antibody [EPR460(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
    Sample
    Mouse Cell (Neural cell)
    Specification
    Neural cell
    Permeabilization
    Yes - 0.3% TritonX-100 in PBS for 30 minutes
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 13 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC1 antibody [EPR460(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted May 31 2012

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