Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Average
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (heart)
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: NA934V Anti rabbit IgG HRP linked whole antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Exposure
20 minute(s) and 0 second(s)

Additional data

Additional Notes
enquiry regarding ab109411 [CCE7410925] order number: 170/100476374 10.10.14

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Verified customer

Submitted Jan 13 2015

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