Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-12% Tris-bis glycine gel)
Sample
Mouse Tissue lysate - other (mouse brain tissue)
Specification
mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H&L) Secondary Antibody, Dyl
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 650
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 650
Dilution
1/1000
Detection
Detection method
fluorescent imaging on LiCor
Exposure
1 minute(s) and 0 second(s)
Negative control
cytoplasmic fraction of mouse brain tissue
Additional data
Additional Notes
Protein transfer was verified using Poncea staining (first image). Fluorescent image did not show the HDAC1 band (second image). A second test at 1:500 anti-HDAC1 was performed and results were negative.
Lane 1: ladder
Lane 2: cytoplasmic fraction
Lane 3: nuclear fraction 1
Lane 4: nuclear fraction 2
Lane 5: nuclear fraction 3
Lane 6: nuclear fraction 4
Abcam response
Thank you for your valuable feedback. We are sorry that this product did not work as stated on the datasheet and as such we have issued the customer a refund.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Arjang Salehi
Verified customer
Submitted Mar 26 2015