Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Western blot

15 µg

Reduced Denaturing (4-12% Tris-bis glycine gel)

Mouse Tissue lysate - other (mouse brain tissue)

mouse brain tissue

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

16 hour(s) and 0 minute(s) · Temperature: 4°C

1/1000

Non-Abcam antibody was used: Goat anti-Rabbit IgG (H&L) Secondary Antibody, Dyl
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight­ 650

1/1000

fluorescent imaging on LiCor

1 minute(s) and 0 second(s)

cytoplasmic fraction of mouse brain tissue

Protein transfer was verified using Poncea staining (first image). Fluorescent image did not show the HDAC1 band (second image). A second test at 1:500 anti-HDAC1 was performed and results were negative. Lane 1: ladder Lane 2: cytoplasmic fraction Lane 3: nuclear fraction 1 Lane 4: nuclear fraction 2 Lane 5: nuclear fraction 3 Lane 6: nuclear fraction 4

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