Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Inconclusive
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-12% Tris-bis glycine gel)
Sample
Mouse Tissue lysate - other (mouse brain tissue)
Specification
mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H&L) Secondary Antibody, Dyl
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight­ 650
Dilution
1/1000

Detection

Detection method
fluorescent imaging on LiCor
Exposure
1 minute(s) and 0 second(s)
Negative control
cytoplasmic fraction of mouse brain tissue

Additional data

Additional Notes
Protein transfer was verified using Poncea staining (first image). Fluorescent image did not show the HDAC1 band (second image). A second test at 1:500 anti-HDAC1 was performed and results were negative. Lane 1: ladder Lane 2: cytoplasmic fraction Lane 3: nuclear fraction 1 Lane 4: nuclear fraction 2 Lane 5: nuclear fraction 3 Lane 6: nuclear fraction 4
Abcam response
Thank you for your valuable feedback. We are sorry that this product did not work as stated on the datasheet and as such we have issued the customer a refund.

Dr. Arjang Salehi

Verified customer

Submitted Mar 26 2015

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