Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Excellent
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - nuclear (B cells)
Specification
B cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Bands
Specific: 55 kDa
Exposure
30 second(s)

Additional data

Additional Notes
The last 4 lanes (next to the Molecular Weight Marker ladder) are loaded with cytoplasmic fractions, and the first 4 lanes are loaded with nuclear fractions.

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Submitted Mar 16 2015

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