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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Reviews (1) Submit a question References (3)
ChIC/CUT&RUN sequencing - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Flow Cytometry - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunocytochemistry - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5517(2)] to HDAC1
  • Suitable for: ChIC/CUT&RUN-seq, WB, IHC-P, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-HDAC1 antibody [EPR5517(2)]
    See all HDAC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5517(2)] to HDAC1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ChIC/CUT&RUN-seq, WB, IHC-P, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HDAC1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cell. Flow Cyt: HeLa cell.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIC/CUT&RUN-seq
Use 5µg for 105 cells.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Flow Cyt
1/500.
Notes
ChIC/CUT&RUN-seq
Use 5µg for 105 cells.
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Flow Cyt
1/500.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

  • Tissue specificity

    Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.

  • Post-translational
    modifications

    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp686H12203 antibody
      • GON 10 antibody
      • HD1 antibody
      • HDAC 1 antibody
      • HDAC1 antibody
      • HDAC1_HUMAN antibody
      • Histone deacetylase 1 antibody
      • Reduced potassium dependency yeast homolog like 1 antibody
      • RPD3 antibody
      • RPD3L1 antibody
      see all

    Images

    • ChIC/CUT&RUN sequencing - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      ChIC/CUT&RUN sequencing - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ab285373 105 HeLa cells and 5µg of ab150399 [EPR5517(2)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads.

      Additional screenshots of mapped reads can be downloaded here.

    • Flow Cytometry - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Flow Cytometry - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling HDAC1 with ab150399 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
    • Immunocytochemistry - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunocytochemistry - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HDAC1 with ab150399 at 1/100 (5.17 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2ug/ml dilution.

    • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human breast carcinoma lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245 observed at 37 kDa.

      ab150399 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab150399 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
      secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      All lanes : Anti-HDAC1 antibody [EPR5517(2)] (ab150399) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling HDAC1 with ab150399 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Purified ab150399 at 1/30 dilution (2µg) immunoprecipitating HDAC1 in Jurkat whole cell lysate.
      Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
      Lane 2 (+): ab150399 + Jurkat whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab150399 in Jurkat whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 62 kDa
      Faint band above 62kDa could be Sumoylated HDAC1. (PMID: 28186506)

    • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

      This western blot image is a comparison between ab150399 and a competitor's top cited rabbit polyclonal antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150399 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab150399 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150399 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150399 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150399 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
      Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

    References (3)

    Publishing research using ab150399? Please let us know so that we can cite the reference in this datasheet.

    ab150399 has been referenced in 3 publications.

    • Ma T  et al. HOXA10 promotion of HDAC1 underpins the development of lung adenocarcinoma through the DNMT1-KLF4 axis. J Exp Clin Cancer Res 40:71 (2021). PubMed: 33596966
    • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
    • Dong LH  et al. Histone deacetylase inhibitor potentiated the ability of MTOR inhibitor to induce autophagic cell death in Burkitt leukemia/lymphoma. J Hematol Oncol 6:53 (2013). WB . PubMed: 23866964

    Customer reviews and Q&As

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    Western blot abreview for Anti-HDAC1 antibody [EPR5517(2)]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    12 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

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    Submitted Jun 25 2015

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