Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

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Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
  • Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5517(2)] to HDAC1
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-HDAC1 antibody [EPR5517(2)]
    See all HDAC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5517(2)] to HDAC1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HDAC1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • K562, Jurkat, MCF-7 and HeLa cell lysates; Human testis tissue; MCF-7 cells. IP: Jurkat whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab150399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

    • Tissue specificity

      Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 1 subfamily.

    • Post-translational
      modifications

      Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
      Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • DKFZp686H12203 antibody
        • GON 10 antibody
        • HD1 antibody
        • HDAC 1 antibody
        • HDAC1 antibody
        • HDAC1_HUMAN antibody
        • Histone deacetylase 1 antibody
        • Reduced potassium dependency yeast homolog like 1 antibody
        • RPD3 antibody
        • RPD3L1 antibody
        see all

      Images

      • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
        Lane 3: HeLa cell lysate (20 µg)
        Lane 4: Human breast carcinoma lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245 observed at 37 kDa.

        ab150399 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab150399 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
        secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

      • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        All lanes : Anti-HDAC1 antibody [EPR5517(2)] (ab150399) at 1/1000 dilution

        Lane 1 : K562 cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : MCF7 cell lysate
        Lane 4 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 55 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling HDAC1 with ab150399 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Purified ab150399 at 1/30 dilution (2µg) immunoprecipitating HDAC1 in Jurkat whole cell lysate.
        Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
        Lane 2 (+): ab150399 + Jurkat whole cell lysate.
        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab150399 in Jurkat whole cell lysate.
        VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
        Blocking Buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM/TBST.
        Observed band size: 62 kDa
        Faint band above 62kDa could be Sumoylated HDAC1. (PMID: 28186506)

      • Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
        Lane 3: HeLa cell lysate (20 µg)
        Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

        This western blot image is a comparison between ab150399 and a competitor's top cited rabbit polyclonal antibody.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150399 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab150399 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150399 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150399 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

        Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150399 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-HDAC1 antibody [EPR5517(2)] (ab150399)
        Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

      References (2)

      Publishing research using ab150399? Please let us know so that we can cite the reference in this datasheet.

      ab150399 has been referenced in 2 publications.

      • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
      • Dong LH  et al. Histone deacetylase inhibitor potentiated the ability of MTOR inhibitor to induce autophagic cell death in Burkitt leukemia/lymphoma. J Hematol Oncol 6:53 (2013). WB . PubMed: 23866964

      Customer reviews and Q&As

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      Western blot abreview for Anti-HDAC1 antibody [EPR5517(2)]

       Poor
      Abreviews
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Brain)
      Gel Running Conditions
      Reduced Denaturing (4-20%)
      Loading amount
      12 µg
      Specification
      Brain
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

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      Verified customer

      Submitted Jun 25 2015

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