Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control (ab68436)

Mouse monoclonal HDAC1 antibody [mAbcam 68436]. Validated in WB and tested in Mouse. Cited in 2 publication(s). Independently reviewed in 1 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control
    See all HDAC1 primary antibodies
  • Description

    Mouse monoclonal [mAbcam 68436] to HDAC1 - Nuclear Loading Control
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human HDAC1 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab20434)

  • Positive control

    • This antibody gave a positive signal in IOUD2 Whole Cell Lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab68436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificity

    Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

Images

  • Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control (ab68436) at 5 µg/ml + IOUD2 Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

References

This product has been referenced in:

  • Asadian P  et al. The expression profile of sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) in feline tissues. Vet Immunol Immunopathol 195:7-18 (2018). WB . Read more (PubMed: 29249320) »
  • Remes Lenicov F  et al. Prostaglandin E2 Antagonizes TGF-ß Actions During the Differentiation of Monocytes Into Dendritic Cells. Front Immunol 9:1441 (2018). Read more (PubMed: 29988364) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 24 2010

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