Product nameAnti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control
See all HDAC1 primary antibodies
DescriptionMouse monoclonal [mAbcam 68436] to HDAC1 - Nuclear Loading Control
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human, Orangutan
Synthetic peptide corresponding to Human HDAC1 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in IOUD2 Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Clone numbermAbcam 68436
Our Abpromise guarantee covers the use of ab68436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.
- Information by UniProt
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control (ab68436) at 5 µg/ml + IOUD2 Whole Cell Lysate at 20 µg
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This product has been referenced in:
- Asadian P et al. The expression profile of sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) in feline tissues. Vet Immunol Immunopathol 195:7-18 (2018). WB . Read more (PubMed: 29249320) »
- Remes Lenicov F et al. Prostaglandin E2 Antagonizes TGF-ß Actions During the Differentiation of Monocytes Into Dendritic Cells. Front Immunol 9:1441 (2018). Read more (PubMed: 29988364) »