• Product name
    Anti-HDAC1 antibody, prediluted
    See all HDAC1 primary antibodies
  • Description
    Rabbit polyclonal to HDAC1, prediluted
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Positive control
    • NIH/3T3 cells or Tonsil This antibody gave a positive result when used in the following formaldehydel fixed cell lines: HepG2.



Our Abpromise guarantee covers the use of ab15316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
    • Tissue specificity
      Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
    • Sequence similarities
      Belongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
      Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686H12203 antibody
      • GON 10 antibody
      • HD1 antibody
      • HDAC 1 antibody
      • HDAC1 antibody
      • HDAC1_HUMAN antibody
      • Histone deacetylase 1 antibody
      • Reduced potassium dependency yeast homolog like 1 antibody
      • RPD3 antibody
      • RPD3L1 antibody
      see all


    • ab15316 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15316 at 1/50 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab15316 staining HDAC1 in breast carcinoma by Immunohistochemistry (FFPE-sections).
    • Immunohistochemical analysis of Human vulvar squamous cell cancer, staining HDAC1 with ab15316 at 1/2 dilution.
    • Immunohistochemical analysis of Human vulvar intraepithelial neoplasia, staining HDAC1 with ab15316 at 1/2 dilution.


    This product has been referenced in:
    • Peper JK  et al. HLA ligandomics identifies histone deacetylase 1 as target for ovarian cancer immunotherapy. Oncoimmunology 5:e1065369 (2016). IHC . Read more (PubMed: 27467910) »
    • Samartzis EP  et al. The Expression of Histone Deacetylase 1, But Not Other Class I Histone Deacetylases, Is Significantly Increased in Endometriosis. Reprod Sci N/A:N/A (2013). Human . Read more (PubMed: 23690335) »
    See all 7 Publications for this product

    Customer reviews and Q&As


    Thank you for your message. I can confirm that the alignment of the immunogen for ab15316 HDAC1 antibody with HDAC 2,3,6,and 10 is as follows: HDAC2 54.50% HDAC3 35.70% HDAC6 0% HDAC10 0% These alignments are very low and so I would suggest that it is very unlikely that ab15316 HDAC1 antibody will cross react with these other members of this protein family. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

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