Overview

  • Product name

  • Description

    Rabbit polyclonal to HDAC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 145-409 of Human HDAC2.

  • Positive control

    • 293T, Raji, A431, H1299, HepG2, HeLa, MOLT4 and Mouse brain whole cell lyastes; HeLa cells; Human Cal27 Xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • Tissue specificity

    Widely expressed; lower levels in brain and lung.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

Images

  • Anti-HDAC2 antibody (ab137364) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Anti-HDAC2 antibody (ab137364) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft tissue labelling HDAC2 with ab137364 at 1/100 dilution.
  • Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling HDAC2 with ab137364 (green) at 1/500 dilution. Alpha-tubulin filaments were labeled with an anti-alpha tubulin antibody (red) at 1/2500.

References

This product has been referenced in:

  • Shen Y  et al. ARHGAP4 regulates the cell migration and invasion of pancreatic cancer by the HDAC2/ß-catenin signaling pathway. Carcinogenesis N/A:N/A (2019). Read more (PubMed: 30958531) »
  • Gao J  et al. HDAC3 But not HDAC2 Mediates Visual Experience-Dependent Radial Glia Proliferation in the Developing Xenopus Tectum. Front Cell Neurosci 10:221 (2016). WB, IHC-Fr ; Xenopus laevis . Read more (PubMed: 27729849) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Xenopus tropicalis Tissue sections (olfactory epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE
Permeabilization
Yes - Triton x-100
Specification
olfactory epithelium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 16 2017

Application
Western blot
Sample
Hydractinia echinata Cell lysate - whole cell (Whole animal)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Whole animal
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 29 2016

Application
Western blot
Loading amount
2 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Xenopus laevis Tissue lysate - whole (Tectum from Xenopus Laevis at stage 48)
Specification
Tectum from Xenopus Laevis at stage 48
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Wanhua Shen

Verified customer

Submitted Jul 18 2013

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