Key features and details
- Rabbit polyclonal to HDAC2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HDAC2 antibody
See all HDAC2 primary antibodies
DescriptionRabbit polyclonal to HDAC2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 145-409 of Human HDAC2.
- 293T, Raji, A431, H1299, HepG2, HeLa, MOLT4 and Mouse brain whole cell lyastes; HeLa cells; Human Cal27 Xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Recombinant Human HDAC2 protein (ab101662)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Mouse brain tissue lysate - total protein (ab30151)
- Recombinant human HDAC2 protein (ab42630)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- MOLT4 whole cell lysate (ab7912)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab137364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 55 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible.|
|ICC/IF||1/100 - 1/1000.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
Tissue specificityWidely expressed; lower levels in brain and lung.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
- Information by UniProt
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
Anti-HDAC2 antibody (ab137364) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 55 kDa
7.5% SDS PAGE
Anti-HDAC2 antibody (ab137364) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 55 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft tissue labelling HDAC2 with ab137364 at 1/100 dilution.
Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling HDAC2 with ab137364 (green) at 1/500 dilution. Alpha-tubulin filaments were labeled with an anti-alpha tubulin antibody (red) at 1/2500.
ab137364 has been referenced in 3 publications.
- Shen Y et al. ARHGAP4 regulates the cell migration and invasion of pancreatic cancer by the HDAC2/ß-catenin signaling pathway. Carcinogenesis N/A:N/A (2019). PubMed: 30958531
- Gao J et al. HDAC3 But not HDAC2 Mediates Visual Experience-Dependent Radial Glia Proliferation in the Developing Xenopus Tectum. Front Cell Neurosci 10:221 (2016). WB, IHC-Fr ; Xenopus laevis . PubMed: 27729849
- Guo X et al. Subcellular Localization of Class I Histone Deacetylases in the Developing Xenopus tectum. Front Cell Neurosci 9:510 (2015). IHC . PubMed: 26793062