Western blot abreview for Anti-HDAC2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (various cell types - cell lines and human primary)
Loading amount
15 µg
Specification
various cell types - cell lines and human primary
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS

Secondary antibody

Name
Non-Abcam antibody was used: GE (Amersham) ECL Rabbit IgG, HRP-Linked F(ab')2 F
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000

Detection

Detection method
West Pico (Pierce)
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 40, 45 kDa

Additional data

Additional Notes
1: THP-1 human macrophage cell line
2: Keratinocytes -primary human
3: Peripheral Blood Mononuclear Cells - primary human
4: MCF-7 - human breast cancer cell line
5: H9c2 - rat cardiomyoblast cell line
6: Rat primary neonatal cardiac myocyte

Prof. Paul Townsend

Verified customer

Submitted May 01 2008

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