Western blot abreview for Anti-HDAC2 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human colon adenocarcinoma cells)
Loading amount
200000 cells
Specification
Human colon adenocarcinoma cells
Treatment
transfected with plasmids (irrelevant to HDAC2)
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk (in PBSTween20)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
HRP
Exposure
2 second(s)
Bands
Specific: 45 kDa Non-specific: 50, 55 kDa

Additional data

Additional Notes
This antibody was used as a loading control. I have also used it as a loading control for experiments with HeLa cells. The same band patterns were observed (very clean gel, with three bands at approximately the same locations). We believe that the band specific to HDAC2, is the strongest observed, at around 45kD.

Ms. Johanna Smith

Verified customer

Submitted Jul 11 2008

Sign up