Immunohistochemistry (Frozen sections) abreview for Anti-HDAC2 antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, 0.2% Triton X in PBS

Secondary antibody

Name
Non-Abcam antibody was used: donkey anti rabbit, Alexa Fluor
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Antigen unmasking with sodium citrate buffer was necessary to obtain full signal strength (sodium citrate 10mM, pH6)

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Submitted Sep 06 2010

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