Immunocytochemistry/ Immunofluorescence

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
African Green Monkey Cell (Cos7 Monkey Kidney Fibroblast)
Specification
Cos7 Monkey Kidney Fibroblast
Permeabilization
Yes - .3% Triton X100
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Blocking Buffer
Dilution
1/300

Secondary antibody

Name
Non-Abcam antibody was used: Cy3 Jackson Labs
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200

Additional data

Additional Notes
Works Excellent in Cos7 and HeLa cell ICC, WB and IP. Shows a very clean band on western blot at ~60kda.

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Submitted Jun 13 2013

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