Western blot abreview for Anti-HDAC2 antibody

Good
Abreviews
Application
Western blot
Loading amount
22 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (293T, MEL cell)
Specification
293T, MEL cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% skim milk in PBS-T
Dilution
1/2500

Secondary antibody

Dilution
1/20000

Detection

Detection method
ECL prime
Bands
Specific: 55 kDa
Exposure
3 minute(s) and 0 second(s)

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Submitted Jul 03 2013

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