Overview

  • Product name

  • Description

    Goat polyclonal to HDAC2
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Synthetic peptide corresponding to Mouse HDAC2 aa 386-397 (internal sequence). (NP_032255.2).
    Sequence:

    (c)PEDAVHEDSGDE


    Database link: P70288

  • Positive control

    • WB: HEK-293 nuclear lysate. ICC/IF: U-251 MG cells. Flow: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab238251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
Flow Cyt Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • Tissue specificity

    Widely expressed; lower levels in brain and lung.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

Images

  • Anti-HDAC2 antibody (ab238251) at 1 µg/ml + HEK-293 (human epithelial cell line from embryonic kidney) nuclear lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa



    Primary incubation 1 hour at room temperature.

  • Paraformaldehyde-fixed, 0.15% Triton permeabilized U-251 MG (human brain glioma cell line) cells labeling HDAC2 (green) using ab238251 at 10 μg/ml in ICC/IF. Primary incubation 1 hour followed by Alexa Fluor 488 secondary antibody at 2 μg/ml. The nuclear stain is DAPI (blue).

    Negative control: Unimmunized goat IgG at 10 μg/ml followed by Alexa Fluor 488 secondary antibody at 2 μg/ml.

  • Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling HDAC2 (blue line) using ab238251 at 10 μg/ml, followed by Alexa Fluor 488-conjugated secondary antibody at 2 μg/ml.

    IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488-conjugated secondary antibody.

     

     

References

ab238251 has not yet been referenced specifically in any publications.

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