Overview

  • Product name

  • Description

    Rabbit polyclonal to HDAC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Synthetic peptide corresponding to Human HDAC2 (N terminal). (NP_001518.1).
    Database link: Q92769

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
      Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
    • Tissue specificity

      Widely expressed; lower levels in brain and lung.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      modifications

      S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • D10Wsu179e antibody
      • HD 2 antibody
      • HD2 antibody
      • HDAC 2 antibody
      • Hdac2 antibody
      • HDAC2_HUMAN antibody
      • Histone deacetylase 2 (HD2) antibody
      • Histone deacetylase 2 antibody
      • OTTHUMP00000017046 antibody
      • OTTHUMP00000227077 antibody
      • OTTHUMP00000227078 antibody
      • RPD3 antibody
      • transcriptional regulator homolog RPD3 antibody
      • YAF1 antibody
      • YY1 associated factor 1 antibody
      • YY1 transcription factor binding protein antibody
      • Yy1bp antibody
      see all

    Images

    • HDAC2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245305 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative rabbit anti-HDAC2 antibody, at 1 µg/ml.

      Lane 1: ab245305 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    ab245305 has not yet been referenced specifically in any publications.

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