Anti-HDAC2 antibody [HDAC2-62] (ab12169)
Key features and details
- Mouse monoclonal [HDAC2-62] to HDAC2
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-HDAC2 antibody [HDAC2-62]
See all HDAC2 primary antibodies -
Description
Mouse monoclonal [HDAC2-62] to HDAC2 -
Host species
Mouse -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
SGEKTDTKGTKSEQLSNP
with N-terminally added Cysteine conjugated to KLH, corresponding to amino acids 471-488 of Human HDAC2. -
Positive control
- Whole cell extracts from NIH-3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HDAC2-62 -
Isotype
IgG2b -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB | (3) |
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 55 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 55 kDa. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. -
Tissue specificity
Widely expressed; lower levels in brain and lung. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3066 Human
- Entrez Gene: 15182 Mouse
- Omim: 605164 Human
- SwissProt: Q92769 Human
- SwissProt: P70288 Mouse
- Unigene: 3352 Human
- Unigene: 19806 Mouse
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Alternative names
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
see all
Images
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Western Blot using Rabbit anti-HDAC2
Lane 1 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 5 µg
Lane 2 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 2.5 µg
Lane 3 : Negative Control at 5 µg
All lanes : HeLa whole cell extract -
All lanes : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 0.5 µg/ml
Lane 1 : Hek293T cell Lysate
Lane 2 : HeLa cell Lysate
Lane 3 : Jurkat cell Lysate
Lane 4 : K562 cell Lysate
Lane 5 : Neuro-2a cell Lysate
Lane 6 : NIH-3T3 cell Lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase -
Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 1/250 dilution + Human glioblastoma cell line at 120 µg
Secondary
HRP-conjugated Goat anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (75)
ab12169 has been referenced in 75 publications.
- Jevtic Z et al. SMARCA5 interacts with NUP98-NSD1 oncofusion protein and sustains hematopoietic cells transformation. J Exp Clin Cancer Res 41:34 (2022). PubMed: 35073946
- Dufour CR et al. The mTOR chromatin-bound interactome in prostate cancer. Cell Rep 38:110534 (2022). PubMed: 35320709
- Xu 许潇依 X et al. CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease. J Neurosci 42:4594-604 (2022). PubMed: 35501151
- Yao W et al. Activation of BDNF by transcription factor Nrf2 contributes to antidepressant-like actions in rodents. Transl Psychiatry 11:140 (2021). PubMed: 33627628
- Liu Y et al. Long noncoding RNA LINC00518 induces radioresistance by regulating glycolysis through an miR-33a-3p/HIF-1a negative feedback loop in melanoma. Cell Death Dis 12:245 (2021). PubMed: 33664256