Product nameAnti-HDAC2 antibody [HDAC2-62] - ChIP Grade
See all HDAC2 primary antibodies
DescriptionMouse monoclonal [HDAC2-62] to HDAC2 - ChIP Grade
Tested applicationsSuitable for: ChIP, ICC/IF, IHC-Fr, IHC-P, ELISA, IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Dog, Human
- Whole cell extracts from NIH-3T3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityProtein A purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab12169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 24919190|
|IHC-Fr||1/1000. PubMed: 20407577|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 55 kDa.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
Tissue specificityWidely expressed; lower levels in brain and lung.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
- Information by UniProt
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
Chromatin was isolated and pooled from the two PF cortices of a single mouse. ChIP experiments were performed using a minimum of 3 mice per group. Chromatin was sheared in an ice bath by a 25 cycles of 30 sec on/off sonication using the “Bioruptor UCD-20” sonicator. Samples were kept on ice during 30 s between two pulses. An aliquot of precleared chromatin was collected as the input. The samples were incubated overnight at 4°C with 5 µl ab12169. The immunoprecipitated chromatin was analyzed by quantitative PCR with primers designed to amplify short regions of the promoters of genes of interest.
ChIP assay on c-Fos gene promoter using ab12169 antibody. Results show a significant lower enrichment of HDAC 2 in PF cortex of (amyloid precursor protein) APP−/− mouse, Student’s t-test: **p<0.01. IgG was used as negative control. Equal amounts of ChIP and input chromatin were used for qRT-PCR analysis on the c-Fos gene promoter. Enrichment values were normalized to input values and represented the average of three or more mice per experiment. Results are expressed as mean ± SD.
ab12169 staining HDAC2 in a human glioblastoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 (0.1%) in PBS and blocked with 0.5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse monoclonal (1/400) was used as the secondary antibody. Nuclei were counterstained with Hoechst (blue).
Western Blot using Rabbit anti-HDAC2
Lane 1 : Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade (ab12169) at 5 µg
Lane 2 : Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade (ab12169) at 2.5 µg
Lane 3 : Negative Control at 5 µg
All lanes : HeLa whole cell extract
All lanes : Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade (ab12169) at 0.5 µg/ml
Lane 1 : Hek293T cell Lysate
Lane 2 : HeLa cell Lysate
Lane 3 : Jurkat cell Lysate
Lane 4 : K562 cell Lysate
Lane 5 : Neuro-2a cell Lysate
Lane 6 : NIH-3T3 cell Lysate
All lanes : Goat Anti-Mouse IgG-Peroxidase
Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade (ab12169) at 1/250 dilution + Human glioblastoma cell line at 120 µg
HRP-conjugated Goat anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
This product has been referenced in:
- Joung H et al. Sumoylation of histone deacetylase 1 regulates MyoD signaling during myogenesis. Exp Mol Med 50:e427 (2018). Read more (PubMed: 29328071) »
- de la Fuente Revenga M et al. Chronic clozapine treatment restrains via HDAC2 the performance of mGlu2 receptor agonism in a rodent model of antipsychotic activity. Neuropsychopharmacology N/A:N/A (2018). Read more (PubMed: 30038413) »