• Product name
    Anti-HDAC2 antibody [Y461]
    See all HDAC2 primary antibodies
  • Description
    Rabbit monoclonal [Y461] to HDAC2
  • Host species
  • Specificity
    ab32117 recognises HDAC2.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HDAC2 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: HAP1, A431, Hela and K562 cell lysate and rat brain tissue homogenate. IHC-P: Human breast carcinoma and rat spinal cord tissue. ICC/IF: MCF-7 and wildtype HAP1 cells. Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 55 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/60 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/60.
IHC-Fr 1/500. May require antigen retrieval if fixing frozen section in paraformaldehyde.


  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • Tissue specificity
    Widely expressed; lower levels in brain and lung.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all


  • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: HDAC2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: A431 whole cell lysate (20 µg)
    Lane 4: Hela whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32117 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32117 was shown to specifically react with HDAC2 when HDAC2 knockout samples were used. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE.  ab32117 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 and 1/10000 respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • ab32117 staining HDAC2 in wild-type HAP1 cells (top panel) and HDAC2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab32117 at 1/250 dilution and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • ab32117 staining HDAC2 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

  • All lanes : Anti-HDAC2 antibody [Y461] (ab32117) at 1/1000 dilution

    Lane 1 : Rat brain tissue homogenate at 20 µg
    Lane 2 : Rat brain tissue homogenate at 40 µg
    Lane 3 : Rat brain tissue homogenate, P1 nuclear fraction at 20 µg
    Lane 4 : Rat brain tissue homogenate, P1 nuclear fraction at 40 µg
    Lane 5 : Rat brain tissue homogenate, P2 non-nuclear fraction at 20 µg
    Lane 6 : Rat brain tissue homogenate, P2 non-nuclear fraction at 40 µg

    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa (possible non-specific binding)

    Exposure time: 5 minutes

    See Abreview

  • Anti-HDAC2 antibody [Y461] (ab32117) at 1/2000 dilution + K562 cell lysate

    Predicted band size: 55 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?

  • HDAC2 was immunoprecipitated from 1mg of Hela(Human epithelial cell line from cervix adenocarcinoma)whole cell lysate with ab32117at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab32117 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP),specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.

    Lane 1: Hela whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemical analysis of HDAC2 expression in paraffin embedded human breast carcinoma tissue section, using 1/250 ab32117.
  • Overlay histogram showing HeLa cells stained with ab32117 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32117, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab32117 staining HDAC2 in Rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500 in PBS + 0.2% TritonX + 1% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Antigen unmasking with sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, ph6) was necessary to obtain a good signal. The sections were counterstained with DAPI.

    See Abreview


This product has been referenced in:
  • Zhang L  et al. Weighted gene co-expression network analysis and connectivity map identifies lovastatin as a treatment option of gastric cancer by inhibiting HDAC2. Gene 681:15-25 (2019). Read more (PubMed: 30266498) »
  • Qi Z  et al. Four Novel Dammarane-Type Triterpenoids from Pearl Knots of Panax ginseng Meyer cv. Silvatica. Molecules 24:N/A (2019). Read more (PubMed: 30909565) »
See all 36 Publications for this product

Customer reviews and Q&As

1-8 of 8 Q&A


Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1211207.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Thank you for your telephone call this afternoon. I am sorry to hear that the current lot of ab32117 is not working so well for you.

I appreciate the time you have spent on these experiments, and it is regrettable the results have not been successful. As the first vial purchased worked well in the same experiments, it seems on this occasion you have regrettably received a bad vial.

I would like to offer a free of charge replacement or credit note in compensation (providing the product has been purchased in the last 6 months). In order to arrange this, I would appreciate if you could confirm your order number and date of purchase?

I would also appreciate if you are able to provide any images from the vial that had worked, and the one that has not. This will be very helpful for our quality monitoring and investigations.

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Thank you for your call today.

At this point I haven't found a protocol for using whole blood in Western blot. Some of the resources I've found (linked below) indicate that the high content of hemoglobin and other proteins in whole blood, red blood cells, and leukocytes/plateletsinterfere with antibody binding.



Is there a particular cell type that you are hoping to analyze? I might be able to find a reference for analyzing these cells in Western blot.

I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you.

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Thanks you for your email. I will look forward receiving the results soon.

Let me know if this antibody show multiple bands with normal positive control lysates.

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Thank you very much for your email.

I have thoroughly checked the protocol again, which looks fine to me. The nuclear extraction kit also have protease inhibitors initso these should also not be a problem.

As per your feedback the antibody works well in Immunohistochemistry so I am little unsure about the cause of multiple bands at the moment. We unfortunately also not have rat specific images so I am sorry I am unable to do anycomparison ofresults.

I would suggest usingstandard lysates of rat dorsal horn tissues (with RIPA buffer lysis) andK562 cell lysates as positive control. This way you can compare the data we have on the datasheet and alsoin the related publications.

I am sure the antibody will be fine. if in case the multiple bands don't go away even after trying the positive control, please let me know I will be happy to assist further.

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Thank you very much for your email. I am very sorry to hear that you have been observing multiple bands with this antibody.

I am currently searching the cause of this anomaly in publications. There is one publication which shows similar pattern of bands Fig 7. however I need to do thorough search to reach a conclusion. http://www.jbc.org/content/278/43/42560.full

The current data we have for this antibody show it should detect a single band at 55 kDa so I am now interested in searching publication if somebody else has got similar results. There is one antibody from different vendor which show multiple bands in rat kidney lysates same as you observed so these may be the splice variants or other form of HDAC.

I will get back to you with the proper explanation soon.

I have few more questions regarding the protocol

- Have you added protease inhibitors when preparing the nuclear lysates? DO you know if the kits has protease inhibitors in it?
- At what temperature the extract were heated with sample buffer (LDS) and for how long?

I will look forward to hearing from you soon.

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

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Thank you for your inquiry.

I heard back from the supplying laboratory that there was an error on our datasheet and that this antibody is provided in cell culture supernatant and
storage buffer, so it is not IgG fraction and purified.

I apologize for the confusion, but thank you for bringing this to our attention.

Please let me know if you have any other questions.

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