Key features and details
- Rabbit polyclonal to HDAC2 (phospho S394)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-HDAC2 (phospho S394) antibody
See all HDAC2 primary antibodies
DescriptionRabbit polyclonal to HDAC2 (phospho S394)
Specificityab75602 detects endogenous levels of HDAC2 only when phosphorylated at serine 394.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D).
- Extracts from HT-29 cells treated with UV (20min). Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75602 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab75602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|WB||1/500 - 1/1000. Predicted molecular weight: 55 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
Tissue specificityWidely expressed; lower levels in brain and lung.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
- Information by UniProt
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
All lanes : Anti-HDAC2 (phospho S394) antibody (ab75602) at 1/500 dilution
Lane 1 : Extracts from HT-29 cells (untreated)
Lane 2 : Extracts from HT-29 cells treated with UV (20min)
Predicted band size: 55 kDa
Observed band size: 55 kDa
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75602, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
ab75602 at 1/100 dilution, staining HDAC2 (phospho S394) in human Hela cells by ICC/IF.
ab75602 has been referenced in 5 publications.
- Shang L et al. Plantamajoside attenuates isoproterenol-induced cardiac hypertrophy associated with the HDAC2 and AKT/ GSK-3ß signaling pathway. Chem Biol Interact 307:21-28 (2019). PubMed: 31009642
- Mattioli E et al. Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS. Aging Cell 17:e12824 (2018). PubMed: 30109767
- Yoon S et al. PP2A negatively regulates the hypertrophic response by dephosphorylating HDAC2 S394 in the heart. Exp Mol Med 50:83 (2018). PubMed: 30050113
- Kobayashi Y et al. Passive smoking impairs histone deacetylase-2 in children with severe asthma. Chest 145:305-12 (2014). Human . PubMed: 24030221
- Khan DH et al. RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing. Nucleic Acids Res N/A:N/A (2013). PubMed: 24234443