Overview

  • Product name

  • Description

    Rabbit polyclonal to HDAC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC - Wholemountmore details
  • Species reactivity

    Reacts with: Zebrafish
    Predicted to work with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant fragment within Zebrafish SDS3 aa 196-428. The exact sequence is proprietary.
    Database link: Q803C3

  • Positive control

    • WB: Whole zebrafish whole cell lysate. IHC-Wm: Zebrafish embryo. IHC-P: Zebrafish tissue.
  • General notes

    (Please refer to the vial label for the specific concentration)

Properties

Applications

Our Abpromise guarantee covers the use of ab209840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95?,1Hr

IHC - Wholemount 1/100 - 1/500.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Sumoylated in vitro.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

Images

  • Anti-HDAC3 antibody (ab209840) at 1/500 dilution + Whole zebrafish whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE

  • Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling HDAC3 with ab209840 at 1/300.

  • Immunohistochemical analysis (whole mount) of Zebrafish embryo labeling HDAC3 with ab209840 at 1/200.

References

ab209840 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab209840.
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