Key features and details
- Rabbit polyclonal to HDAC3
- Suitable for: WB, IHC-P, IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-HDAC3 antibody
See all HDAC3 primary antibodies
DescriptionRabbit polyclonal to HDAC3
Tested applicationsSuitable for: WB, IHC-P, IHC - Wholemountmore details
Species reactivityReacts with: Zebrafish
Predicted to work with: Mouse, Rat, Cow, Human
Recombinant fragment within Zebrafish SDS3 aa 196-428. The exact sequence is proprietary.
Database link: Q803C3
- WB: Whole zebrafish whole cell lysate. IHC-Wm: Zebrafish embryo. IHC-P: Zebrafish tissue.
(Please refer to the vial label for the specific concentration)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab209840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95?,1Hr
|IHC - Wholemount||1/100 - 1/500.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsSumoylated in vitro.
- Information by UniProt
- HD3 antibody
- HDAC 3 antibody
- HDAC3 antibody
Anti-HDAC3 antibody (ab209840) at 1/500 dilution + Whole zebrafish whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling HDAC3 with ab209840 at 1/300.
Immunohistochemical analysis (whole mount) of Zebrafish embryo labeling HDAC3 with ab209840 at 1/200.
ab209840 has not yet been referenced specifically in any publications.