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Inquiry: Dear Scientific Support group, We have been using ab16047 Ab to detect HDAC3 in WB for many years, results are always consistent. I recently performed a ChIP assay using this Ab, but it failed. I know that this Ab is not recommended for ChIP. I found another Ab #ab7030, which is ChIP grade. I also noticed that both of these HDAC3 antibodies are raised against C-terminus of human HDAC3. I was wondering what makes these two Abs so different in terms of ChIP assays, since they both detect the similar region. I was wondering whether you have documented this difference? In addition, which HDAC3 Ab do you recommend for ChIP assay based on your experience? Thanks, Yue
Asked on Nov 15 2012
Thank you for contacting us.
While the specific immunogen for both ab16047 and ab7030 are similar, some antibodies work better in certain applications due to the nature in which they are derived, stored, and their specific affinity for the antigen. Specifically, our "ChIP Grade" antibodies such as the ab7030 that you mentioned have been tested and validated to work specifically in ChIP.
I recommend the HDAC3 ChIP Grade antibody that you referenced (ab7030: https://www.abcam.com/hdac3-antibody-chip-grade-ab7030.html) as it has 10 Abreviews with an average rating of 4/5 stars (2 Abreviews for ChIP) and 20 specific references.
For your convencience, I've included links to the 2 ChIP Abreviews for ab7030 below:
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Nov 15 2012