Overview

  • Product name

    Anti-HDAC3 antibody - C-terminal
    See all HDAC3 primary antibodies
  • Description

    Rabbit polyclonal to HDAC3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 361-428 of the Human HDAC3 protein (O15379).

  • Positive control

    • WB: 293T, A431, H1299, HeLaS3, HepG2,Molt-4, and Raji whole cell lysate. IF: HeLa cells. IHC-P: SW480 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Sumoylated in vitro.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

Images

  • All lanes : Anti-HDAC3 antibody - C-terminal (ab137704) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : Hep G2 whole cell lysate
    Lane 6 : MOLT4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 48 kDa



    10% SDS PAGE
  • Immunohistochemistry analysis of paraffin-embedded SW480 xenograft labelling FGFR3 with ab137704 at 1/500.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling HDAC3 with ab137704 at 1/200.

References

ab137704 has not yet been referenced specifically in any publications.

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