Product nameAnti-HDAC3 antibody - C-terminal
See all HDAC3 primary antibodies
DescriptionRabbit polyclonal to HDAC3 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus tropicalis
Synthetic peptide corresponding to a region within the C terminal amino acids 361-428 of the Human HDAC3 protein (O15379).
- WB: 293T, A431, H1299, HeLaS3, HepG2,Molt-4, and Raji whole cell lysate. IF: HeLa cells. IHC-P: SW480 xenograft.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsSumoylated in vitro.
- Information by UniProt
- HD3 antibody
- HDAC 3 antibody
- HDAC3 antibody
All lanes : Anti-HDAC3 antibody - C-terminal (ab137704) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : Hep G2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE
Immunohistochemistry analysis of paraffin-embedded SW480 xenograft labelling FGFR3 with ab137704 at 1/500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling HDAC3 with ab137704 at 1/200.
ab137704 has not yet been referenced specifically in any publications.