Overview

  • Product name

    Anti-HDAC3 antibody [EP462Y] - BSA and Azide free
    See all HDAC3 primary antibodies
  • Description

    Rabbit monoclonal [EP462Y] to HDAC3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within HDAC3 (N terminal). The exact sequence is proprietary.

  • General notes

    Ab247459 is the carrier-free version of ab76295. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247459 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      modifications

      Sumoylated in vitro.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HD3 antibody
      • HDAC 3 antibody
      • HDAC3 antibody
      • HDAC3_HUMAN antibody
      • Histone deacetylase 3 antibody
      • RPD3 2 antibody
      • RPD3 antibody
      • RPD3-2 antibody
      • SMAP45 antibody
      see all

    References

    ab247459 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab247459.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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