Overview

  • Product name

    Anti-HDAC3 antibody [NOTGIVEN] - C-terminal
    See all HDAC3 primary antibodies
  • Description

    Mouse monoclonal [NOTGIVEN] to HDAC3 - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: ChIPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HDAC3 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: O15379

  • Positive control

    • ChIP: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab231909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.

5 µl/ChIP reaction.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Sumoylated in vitro.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

Images

  • ChIP assays were performed using HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, ab231909 and optimized PCR primer sets for qPCR.

    ChIP was performed on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. Respectively, 5 µl of ab231909 and 5 µg of IgG (negative IP control) were used. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

References

ab231909 has not yet been referenced specifically in any publications.

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