Recombinant Anti-HDAC3 antibody [Y415] (ab32369)
![Western blot - Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369-261837-anti-hdac3-antibody-y415-western-blot.jpg "Western blot - Anti-HDAC3 antibody [Y415] (ab32369)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y415] to HDAC3
- Suitable for: Flow Cyt, Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HDAC3 antibody [Y415]
See all HDAC3 primary antibodies -
Description
Rabbit monoclonal [Y415] to HDAC3 -
Host species
Rabbit -
Specificity
This antibody may detect splice isoform 2 (RPD3-2A). -
Tested applications
Suitable for: Flow Cyt, Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human HDAC3 (C terminal). The exact sequence is proprietary.
(Peptide available asab221015) -
Positive control
- K562 cell lysate; HeLa cells; human ovary carcinoma
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.12% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y415 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/30.
|
|
| Flow Cyt (Intra) |
1/30.
|
|
| WB | (3) |
1/5000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
|
| IHC-P | (3) |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500. |
| ICC/IF |
1/250 - 1/500.
|
|
| IP |
1/20 - 1/50.
|
| Notes |
|---|
|
Flow Cyt
1/30. |
|
Flow Cyt (Intra)
1/30. |
|
WB
1/5000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
|
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500. |
|
ICC/IF
1/250 - 1/500. |
|
IP
1/20 - 1/50. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated in vitro. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 8841 Human
- Entrez Gene: 15183 Mouse
- Entrez Gene: 84578 Rat
- Omim: 605166 Human
- SwissProt: O15379 Human
- SwissProt: O88895 Mouse
- SwissProt: Q6P6W3 Rat
- Unigene: 519632 Human
see all -
Alternative names
- HD3 antibody
- HDAC 3 antibody
- HDAC3 antibody
see all
Images
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Western blot - Anti-HDAC3 antibody [Y415] (ab32369)All lanes : Anti-HDAC3 antibody [Y415] (ab32369) at 1/5000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 4 : C6 (rat glioma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369-261847-anti-hdac3-antibody-y415-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (ab32369)Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma sections labelling HDAC3 with purified ab32369 at dilution of 1:50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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![Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369-261851-anti-hdac3-antibody-y415-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (ab32369)Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling HDAC3 with purified ab32369 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor®594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).
For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used and for negative control 2, mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) were used.
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![Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369-270693-anti-hdac3-antibody-y415-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] (ab32369)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HDAC3 with purified ab32369 at 1/30 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369-44587-anti-hdac3-antibody-y415-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (ab32369)Immunofluorescent staining of HeLa cells
using ab32369 at 1/250 dilution -
![Immunoprecipitation - Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369-261860-anti-hdac3-antibody-y415-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HDAC3 antibody [Y415] (ab32369)Purified ab32369 at 1/20 immunoprecipitating HDAC3 in K562 whole cell lysate observed at 49 KDa (lanes 1 and 2).
Lane 1 (input): K562 whole cell lysate 10ug
Lane 2 (+): ab32369 + K562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32369 in K562 whole cell lysate
The secondary antibody used was VeriBlot for IP Detection Reagent (HRP) (ab131366) for detection at dilution of 1/1000.
Blocking/Diluting buffer 5% NFDM/TBST.
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![Western blot - Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369WB.bmp)
Western blot - Anti-HDAC3 antibody [Y415] (ab32369)Anti-HDAC3 antibody [Y415] (ab32369) at 1/10000 dilution + K562 cell lysate
Predicted band size: 49 kDa
Observed band size: 49 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369-44588-anti-hdac3-antibody-y415-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (ab32369)Paraffin-embedded human ovary carcinoma
ab32369 at 1/250 dilutionPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-HDAC3 antibody [Y415] (ab32369)](https://www.abcam.com/ps/products/32/ab32369/Images/ab32369-9-benefits-of-recombinant-antibodies.png)
Anti-HDAC3 antibody [Y415] (ab32369)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab32369 has been referenced in 33 publications.
- Yang C et al. FBP1 binds to the bromodomain of BRD4 to inhibit pancreatic cancer progression. Am J Cancer Res 10:523-535 (2020). PubMed: 32195024
- Bao Y et al. Advanced glycation end products inhibit neural stem cell differentiation via upregualtion of HDAC3 expression. Brain Res Bull 159:1-8 (2020). PubMed: 32142834
- Cao L et al. Genotoxic stress-triggered ß-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis. Nat Commun 10:3761 (2019). PubMed: 31434880
- Du Y et al. Ubiquitin specific peptidase 5 promotes ovarian cancer cell proliferation through deubiquitinating HDAC2. Aging (Albany NY) 11:9778-9793 (2019). PubMed: 31727867
- Gilbert TM et al. Neuroepigenetic signatures of age and sex in the living human brain. Nat Commun 10:2945 (2019). PubMed: 31270332