Key features and details
- Rabbit polyclonal to HDAC3 (phospho S424)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HDAC3 (phospho S424) antibody
See all HDAC3 primary antibodies
DescriptionRabbit polyclonal to HDAC3 (phospho S424)
Specificityab61056 detects endogenous levels of HDAC3 only when phosphorylated at serine 424.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide derived from human HDAC3 around the phosphorylation site of serine 424 (KESpDV).
- NIH/3T3 cell extract.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab61056 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab61056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsSumoylated in vitro.
- Information by UniProt
- HD3 antibody
- HDAC 3 antibody
- HDAC3 antibody
ab61056 has not yet been referenced specifically in any publications.