Overview

  • Product name

    Anti-HDAC4 + 5 + 9 antibody
  • Description

    Rabbit polyclonal to HDAC4 + 5 + 9
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of total HDAC4 + 5 + 9
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to amino acids around 244-248/257-261/218-222 of Human HDAC4 + 5 + 9 (NP_006028.2 NP_001015053.1 NP_055522.1)

  • Positive control

    • Methanol-fixed HeLa cells, 293 and HeLa cell lysates, Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 119, 122, 111 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Relevance

    HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. HDAC5: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. HDAC9: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • Cellular localization

    HDAC4: Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation. HDAC5: Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1. HDAC9: Nucleus
  • Database links

  • Alternative names

    • AHO3 antibody
    • Antigen NY-CO-9 antibody
    • BDMR antibody
    • HA6116 antibody
    • HD4 antibody
    • HD5 antibody
    • HD7 antibody
    • HD7b antibody
    • HD9 antibody
    • HDAC antibody
    • HDAC-4 antibody
    • HDAC-A antibody
    • HDAC4 antibody
    • HDAC5 antibody
    • HDAC7 antibody
    • HDAC7B antibody
    • HDAC9 antibody
    • HDAC9B antibody
    • HDAC9FL antibody
    • HDACA antibody
    • HDRP antibody
    • Histone deacetylase 4 antibody
    • Histone deacetylase 5 antibody
    • Histone deacetylase 7B antibody
    • Histone deacetylase 9 antibody
    • Histone deacetylase-related protein antibody
    • MEF2-interacting transcription repressor MITR antibody
    • MITR antibody
    • NY-CO-9 antibody
    see all

Images

  • All lanes : Anti-HDAC4 + 5 + 9 antibody (ab131524) at 1/500 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate

    Predicted band size: 119, 122, 111 kDa

  • Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab131524 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelled with ab131524 at 1/50 dilution (left) or the same antibody preincubated with blocking peptide (right).

References

ab131524 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Drosophila melanogaster Tissue (Axons)
Specification
Axons

Abcam user community

Verified customer

Submitted Jan 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (rhabdomyosarcoma)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K, 37C, 5min
Permeabilization
No
Specification
rhabdomyosarcoma
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 30 2016

Answer

Thank you for your enquiry.

I can confirm that the immunogen for ab131524 has 100% alignment to the drosophila HDAC4 protein. It is therefore likely to detect HDAC4 in drosophila .However, please note we are not able to provide our guarantee for this without further testing. The antibody may also detect Drosophila HDAC5 and 6 aswel.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the individual datasheet, or we will offer a replacement, credit, or refund within 12 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

www.abcam.com/abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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