Anti-HDAC4 antibody [7E2E6] (ab234084)
Key features and details
- Mouse monoclonal [7E2E6] to HDAC4
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HDAC4 antibody [7E2E6]
See all HDAC4 primary antibodies -
Description
Mouse monoclonal [7E2E6] to HDAC4 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human HDAC4 aa 456-592. Expressed in E.coli.
Database link: P56524 -
Positive control
- WB: Human HDAC4 (aa 456-592) recombinant protein; HDAC4 (aa 456-592)-hIgGFc-transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7E2E6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/500.
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Flow Cyt |
1/200 - 1/400.
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ICC/IF |
1/200 - 1/1000.
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Notes |
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WB
1/100 - 1/500. |
Flow Cyt
1/200 - 1/400. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsPhosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation. - Information by UniProt
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Database links
- Entrez Gene: 9759 Human
- Omim: 605314 Human
- SwissProt: P56524 Human
- Unigene: 20516 Human
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Alternative names
- AHO3 antibody
- BDMR antibody
- EC 3.5.1.98 antibody
see all
Images
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Anti-HDAC4 antibody [7E2E6] (ab234084) at 1/100 dilution + Human HDAC4 (aa 456-592) recombinant protein.
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Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling HDAC4 using ab234084 at 1/200 dilution (green).
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments labeled with AlexaFluor®-555-Phalloidin.
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All lanes : Anti-HDAC4 antibody [7E2E6] (ab234084) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : HDAC4 (aa 456-592)-hIgGFc-transfected HEK-293 cell lysate. -
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinom) cells labeling HDAC4 using ab234084 at 1/200 dilution (green) compared to a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab234084 has been referenced in 2 publications.
- Wen Y et al. circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells. Oncol Rep 45:169-179 (2021). PubMed: 33416147
- Wang X et al. Long Noncoding RNA RGMB-AS1 Acts as a microRNA-574 Sponge Thereby Enhancing the Aggressiveness of Gastric Cancer via HDAC4 Upregulation. Onco Targets Ther 13:1691-1704 (2020). PubMed: 32158233