Overview

  • Product name

    Anti-HDAC4 antibody [7E2E6]
    See all HDAC4 primary antibodies
  • Description

    Mouse monoclonal [7E2E6] to HDAC4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human HDAC4 aa 456-592. Expressed in E.coli.
    Database link: P56524

  • Positive control

    • WB: Human HDAC4 (aa 456-592) recombinant protein; HDAC4 (aa 456-592)-hIgGFc-transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab234084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain

    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications

    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Images

  • Anti-HDAC4 antibody [7E2E6] (ab234084) at 1/100 dilution + Human HDAC4 (aa 456-592) recombinant protein.
  • Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling HDAC4 using ab234084 at 1/200 dilution (green).

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments labeled with AlexaFluor®-555-Phalloidin.

  • All lanes : Anti-HDAC4 antibody [7E2E6] (ab234084) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
    Lane 2 : HDAC4 (aa 456-592)-hIgGFc-transfected HEK-293 cell lysate.
  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinom) cells labeling HDAC4 using ab234084 at 1/200 dilution (green) compared to a negative control (red).

References

ab234084 has not yet been referenced specifically in any publications.

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