Anti-HDAC4 antibody (ab12172)
Key features and details
- Rabbit polyclonal to HDAC4
- Suitable for: ICC/IF, IHC-P, WB, ICC, IP, Electron Microscopy
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
Overview
-
Product name
Anti-HDAC4 antibody
See all HDAC4 primary antibodies -
Description
Rabbit polyclonal to HDAC4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ICC, IP, Electron Microscopymore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human
Predicted to work with: Chicken
-
Immunogen
Synthetic peptide:
MSSQSHPDGLSGRDQPVEL
, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken. -
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before useThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 21953612
|
| WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 119 kDa.
Additional weak bands may be detected when blotting using different extract preparations. |
| ICC |
1/250.
|
|
| IP |
Use at an assay dependent concentration.
10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. |
|
| Electron Microscopy |
Use at an assay dependent concentration. PubMed: 18250163
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 21953612 |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 119 kDa. Additional weak bands may be detected when blotting using different extract preparations. |
|
ICC
1/250. |
|
IP
Use at an assay dependent concentration. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. |
|
Electron Microscopy
Use at an assay dependent concentration. PubMed: 18250163 |
Target
-
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsPhosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation. - Information by UniProt
-
Database links
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
see all -
Alternative names
- AHO3 antibody
- BDMR antibody
- EC 3.5.1.98 antibody
see all
Images
-
Immunocytochemistry - Anti-HDAC4 antibody (ab12172)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hela tissue labelling HDAC4 with ab12172 at 1/250 dilution.
-
Immunoprecipitation - Anti-HDAC4 antibody (ab12172)Anti-HDAC4 antibody, ab12172, was used to immunoprecipitate HDAC4 from HeLa whole cell extract.Lane 1: 10 µg of ab12172Lane 2: 5 µg of ab12172Negative control: No cell sextractPositive control: HeLa whole cell extra
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (21)
ab12172 has been referenced in 21 publications.
- Wei W et al. HDAC4 induces the development of asthma by increasing Slug-upregulated CXCL12 expression through KLF5 deacetylation. J Transl Med 19:258 (2021). PubMed: 34118928
- Sato T et al. A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction. Nat Commun 11:3282 (2020). PubMed: 32612176
- Liu J et al. Knockdown of CircCRIM1 Inhibits HDAC4 to Impede Osteosarcoma Proliferation, Migration, and Invasion and Facilitate Autophagy by Targeting miR-432-5p. Cancer Manag Res 12:10199-10210 (2020). PubMed: 33116874
- Gondaliya P et al. miR-29b attenuates histone deacetylase-4 mediated podocyte dysfunction and renal fibrosis in diabetic nephropathy. J Diabetes Metab Disord 19:13-27 (2020). PubMed: 32550152
- Schader T et al. Oxidation of HDAC4 by Nox4-derived H2O2 maintains tube formation by endothelial cells. Redox Biol 36:101669 (2020). PubMed: 32818796