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    hdac4-antibody-ab12172.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-HDAC4 antibody (ab12172)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (15)

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Immunocytochemistry - Anti-HDAC4 antibody (ab12172)
  • Immunoprecipitation - Anti-HDAC4 antibody (ab12172)

Key features and details

  • Rabbit polyclonal to HDAC4
  • Suitable for: ICC, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HDAC4 antibody
    See all HDAC4 primary antibodies
  • Description

    Rabbit polyclonal to HDAC4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Human
    IP
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    MSSQSHPDGLSGRDQPVEL

    , corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human HDAC4 protein (ab104029)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab12172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IP
Human
All applications
Chicken
Application Abreviews Notes
ICC
1/250.
IP
Use at an assay dependent concentration.
Notes
ICC
1/250.
IP
Use at an assay dependent concentration.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain

    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications

    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Target information above from: UniProt accession P56524 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9759 Human
    • Omim: 605314 Human
    • SwissProt: P56524 Human
    • Unigene: 20516 Human
    • Alternative names

      • AHO3 antibody
      • BDMR antibody
      • EC 3.5.1.98 antibody
      • HA6116 antibody
      • HD 4 antibody
      • HD4 antibody
      • HDAC 4 antibody
      • HDAC A antibody
      • HDAC4 antibody
      • HDAC4_HUMAN antibody
      • HDACA antibody
      • Histone deacetylase 4 antibody
      • Histone Deacetylase A antibody
      • KIAA0288 antibody
      see all

    Images

    • Immunocytochemistry - Anti-HDAC4 antibody (ab12172)
      Immunocytochemistry - Anti-HDAC4 antibody (ab12172)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hela tissue labelling HDAC4 with ab12172 at 1/250 dilution. 

    • Immunoprecipitation - Anti-HDAC4 antibody (ab12172)
      Immunoprecipitation - Anti-HDAC4 antibody (ab12172)
      Anti-HDAC4 antibody, ab12172, was used to immunoprecipitate HDAC4 from HeLa whole cell extract.
      Lane 1: 10 µg of ab12172
      Lane 2: 5 µg of ab12172
      Negative control: No cell sextract
      Positive control: HeLa whole cell extra

       

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (15)

    Publishing research using ab12172? Please let us know so that we can cite the reference in this datasheet.

    ab12172 has been referenced in 15 publications.

    • Nishimori S  et al. PTHrP targets HDAC4 and HDAC5 to repress chondrocyte hypertrophy. JCI Insight 4:N/A (2019). PubMed: 30843886
    • Sun N  et al. Effects of histone deacetylase inhibitor Scriptaid and parathyroid hormone on osteocyte functions and metabolism. J Biol Chem 294:9722-9733 (2019). PubMed: 31068415
    • Li C  et al. Nuclear receptor corepressor 1 represses cardiac hypertrophy. EMBO Mol Med 11:e9127 (2019). PubMed: 31532577
    • Cao K  et al. Histone Deacetylase 4 Promotes Osteosarcoma Cell Proliferation and Invasion by Regulating Expression of Proliferating Cell Nuclear Antigen. Front Oncol 9:870 (2019). PubMed: 31552187
    • Yano M  et al. Association of histone deacetylase expression with histology and prognosis of ovarian cancer. Oncol Lett 15:3524-3531 (2018). PubMed: 29456726
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-HDAC4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Bos taurus Cell lysate - whole cell (Skeletal muscle cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% bis tris gel)
    Loading amount
    20 µg
    Specification
    Skeletal muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Chansik Yoon

    Verified customer

    Submitted Oct 14 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC4 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Embryonic (E14.5) hearth)
    Permeabilization
    No
    Specification
    Embryonic (E14.5) hearth
    Blocking step
    TSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HDAC4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (rhabdomyosarcoma)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K, 5 min, 37C
    Permeabilization
    No
    Specification
    rhabdomyosarcoma
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 30 2016

    Western blot abreview for Anti-HDAC4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10%SDS gel)
    Sample
    Mouse Tissue lysate - whole (hippocampus)
    Specification
    hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 30 2015

    Western blot abreview for Anti-HDAC4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (9% gel)
    Sample
    Rat Cell lysate - whole cell (hippocampus)
    Specification
    hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 30 2013

    Western blot abreview for Anti-HDAC4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Hippocampus)
    Loading amount
    15 µg
    Specification
    Hippocampus
    Gel Running Conditions
    Reduced Denaturing (10% SDS gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Ms. Seungyeon Ko

    Verified customer

    Submitted May 14 2013

    Western blot abreview for Anti-HDAC4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Cell lysate - whole cell (hippocampus)
    Loading amount
    25 µg
    Specification
    hippocampus
    Gel Running Conditions
    Reduced Denaturing (10%SDS gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Miss. Sung-Eun WANG

    Verified customer

    Submitted Apr 17 2013

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