Anti-HDAC4 antibody (ab12172)
- Datasheet
- References (10)
- Protocols
Overview
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Product nameAnti-HDAC4 antibody
See all HDAC4 primary antibodies -
DescriptionRabbit polyclonal to HDAC4
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, Electron Microscopymore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Predicted to work with: Chicken
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Immunogen
Synthetic peptide:
MSSQSHPDGLSGRDQPVEL
, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken. -
Positive control
- Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cell (Western blotting) and HeLa nuclei (immunoprecipitation).
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General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody is affinity-purified using the immunizing peptide immobilized on agarose.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab12172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 140 kDa. Additional weak bands may be detected when blotting using different extract preparations. | |
| IP | Use at an assay dependent concentration. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 21953612 | |
| Electron Microscopy | Use at an assay dependent concentration. PubMed: 18250163 |
Target
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FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
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Tissue specificityUbiquitous.
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Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
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Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
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DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
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Post-translational
modificationsPhosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. -
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
- Information by UniProt
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Database links
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
see all -
Alternative names
- AHO3 antibody
- BDMR antibody
- EC 3.5.1.98 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 antibody (ab12172)This image is courtesy of an anonymous abreview.ab12172 staining HDAC4 in human rhabdomyosarcoma (soft tissue sarcoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% H2O2 for 10 minutes at 25°C; antigen retrieval was enzymatic using a proteinase K buffer for 5 minutes at 37oC. Samples were incubated with primary antibody (1/200) for 25 minutes at 25°C. An undiluted HRP-conjugated goat anti-mouse/rabbit IgG polyclonal was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Yano M et al. Association of histone deacetylase expression with histology and prognosis of ovarian cancer. Oncol Lett 15:3524-3531 (2018). Read more (PubMed: 29456726) »
- Wang SE et al. Capsaicin upregulates HDAC2 via TRPV1 and impairs neuronal maturation in mice. Exp Mol Med 50:e455 (2018). Read more (PubMed: 29520110) »
