Overview

  • Product name

    Anti-HDAC4 antibody [EPR22937-157]
    See all HDAC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR22937-157] to HDAC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human HDAC4 aa 1-300. The exact sequence is proprietary.
    Database link: P56524

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate, HEK-293T, HeLa, A549, Neuro-2a, RAW 264.7 and NIH/3T3 lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
    • Tissue specificity

      Ubiquitous.
    • Involvement in disease

      Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Domain

      The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
    • Post-translational
      modifications

      Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
      Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
    • Information by UniProt
    • Database links

    • Alternative names

      • AHO3 antibody
      • BDMR antibody
      • EC 3.5.1.98 antibody
      • HA6116 antibody
      • HD 4 antibody
      • HD4 antibody
      • HDAC 4 antibody
      • HDAC A antibody
      • HDAC4 antibody
      • HDAC4_HUMAN antibody
      • HDACA antibody
      • Histone deacetylase 4 antibody
      • Histone Deacetylase A antibody
      • KIAA0288 antibody
      see all

    Images

    • All lanes : Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : HDAC4 knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 119 kDa
      Observed band size: 140 kDa
      why is the actual band size different from the predicted?



      ab235583 was shown to specifically react with HDAC4 in wild-type HAP1 cells as signal was lost in HDAC4 knockout cells. Wild-type and HDAC4 knockout samples were subjected to SDS-PAGE.

      ab235583 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc MP instrument using the ECL technique.

      Exposure time: 3 minutes.

      Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

      Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 119 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?



      Exposure time: 3 minutes.

      Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
      Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 119 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?



      Lanes 3-4 in this blot were developed using a higher sensitivity ECL substrate.

      Exposure time: Lanes 1-2: 81 seconds Lane 3: 48 seconds Lane 4: 15 seconds.

      Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    References

    ab235583 has not yet been referenced specifically in any publications.

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