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Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Our Abpromise guarantee covers the use of ab12171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa).|
|ChIP||Use at an assay dependent concentration. PubMed: 21586557|
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: HDAC4 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: NIH3T3 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab12171 observed at 140 kDa. Red - loading control, ab176560, observed at 52 kDa.
ab12171 was shown to recognize HDAC4 when HDAC4 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC4 knockout samples were subjected to SDS-PAGE. Ab12171 and ab176560 (loading control to alpha tubulin) were diluted at 1 µg/ml and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
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