Product nameAnti-HDAC4 antibody [HDAC-144] - ChIP Grade
See all HDAC4 primary antibodies
DescriptionMouse monoclonal [HDAC-144] to HDAC4 - ChIP Grade
Tested applicationsSuitable for: ELISA, ICC, IP, WB, ChIPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
- WB: HeLa, NIH/3T3, P19, Jurkat and PC-12 cell lysates; Rat brain lysate. Total cell extracts of NIH3T3 fibroblast cells.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesPurified from ascites.
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class II / Hda1 Class
ChIP Related Products
Our Abpromise guarantee covers the use of ab12171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa).|
|ChIP||Use at an assay dependent concentration. PubMed: 21586557|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
modificationsPhosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
- Information by UniProt
- AHO3 antibody
- BDMR antibody
- EC 126.96.36.199 antibody
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: HDAC4 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: NIH3T3 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab12171 observed at 140 kDa. Red - loading control, ab176560, observed at 52 kDa.
ab12171 was shown to recognize HDAC4 when HDAC4 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC4 knockout samples were subjected to SDS-PAGE. Ab12171 and ab176560 (loading control to alpha tubulin) were diluted at 1 µg/ml and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-HDAC4 antibody [HDAC-144] - ChIP Grade (ab12171) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 3 : P19 cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 5 : Rat brain lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
All lanes : Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 119 kDa
This product has been referenced in:
- Matsuda KI et al. Histone Deacetylation during Brain Development Is Essential for Permanent Masculinization of Sexual Behavior. Endocrinology 152:2760-7 (2011). WB, ChIP ; Rat . Read more (PubMed: 21586557) »
- Kozhemyakina E et al. PTHrP represses chondrocyte hypertrophy through a PP2A/HDAC4/MEF2 pathway. Mol Cell Biol : (2009). Read more (PubMed: 19704004) »