Anti-HDAC4 (phospho S632) antibody (ab39408)


  • Product name
    Anti-HDAC4 (phospho S632) antibody
    See all HDAC4 primary antibodies
  • Description
    Rabbit polyclonal to HDAC4 (phospho S632)
  • Host species
  • Specificity
    ab39408 detects endogenous levels of HDAC4 only when phosphorylated at serine 632.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of serine 632 (A-Q-SP-S-P)



Our Abpromise guarantee covers the use of ab39408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 119 kDa.
ELISA 1/20000.


  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificity
  • Involvement in disease
    Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain
    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHO3 antibody
    • BDMR antibody
    • EC antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all


  • All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution

    Lane 1 : Extract from Jurkat cells (5-30ug of total protein)
    Lane 2 : Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)

    Predicted band size: 119 kDa

  • 20 µg of nuclear lysate prepared from mouse liver was diluted in SDS buffer and run on SDS-PAGE gel. Samples were transferred to nitrocellulose overnight, and blocked with 5% BSA for 8 hours. They were then incubated overnight with primary antibodies, which included an anti-HDAC1, 1/1000 and ab39408, 1/1000. After washing, membranes were incubated with horseradish peroxidase-coupled rabbit or mouse secondary antibodies (1/5000 to 1/10,000) in 5% milk for 1 hour and developed with a Super Signal West Pico chemiluminescent kit.
  • All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution

    Lane 1 : Lysate from murine heart tissue 1
    Lane 2 : Lysate from murine heart tissue 2

    Lysates/proteins at 20 µg per lane.

    All lanes : HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 119 kDa
    Observed band size: 130 kDa (why is the actual band size different from the predicted?)

    Exposure time: 1 minute


This product has been referenced in:
  • Amara S  et al. Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS One 12:e0180097 (2017). Read more (PubMed: 28658303) »
  • Popescu I  et al. Elevated local [Ca2+] and CaMKII promote spontaneous Ca2+ release in ankyrin-B-deficient hearts. Cardiovasc Res 111:287-94 (2016). Read more (PubMed: 27131508) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you very much for your reply.
It looks like there may be a band around the expected molecular weight that is more visible in the even lanes compared to the odd lanes (except for lane 9, it looks like the band is also in this lane). However i...

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Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (5)
Rat Cell lysate - whole cell (Hippocampal primary Neurons)
Hippocampal primary Neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (Mouse brain)
Loading amount
20 µg
Mouse brain
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 01 2013

Thank you for your reply. It is definitely possible that you may notice a shift in the molecular weight. We have not tested this extensively with our general HDAC5 antibodies, but it could explain the extra bands in lane three of the WB image for ab119...

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Thank you very much for your reply and for sending this additional information.
I do have a couple more questions. What gene is being transduced in these L6 cells? Were the Western blot results similar in the control cells?
We have not yet te...

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Thank you for contacting us and letting us know about the trouble with ab53693.
I do have a few questions so that I can better understand the situation:
1) What kind of samples were used? What species and cell/tissue type? Were they treated t...

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Thank you for contacting us. We have only tested this antibody in HeLa cells for IF. The results were not very convincing, but if your customer is still interested in testing it with a different cell line, I can offer him/her a testing discount to perf...

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Thank you for your phone call the other day and for your patience while I have been in touch with the lab about ab39408. For the positive control Jurkat cells, the following protocol was used: A. Jurkat cells were plated on flask or dishes conta...

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Thank you for your enquiry. I have contacted the originator of the product but unfortunately, they do not know of a good positive control for this product. However, the product has been tested in Jurkat cells treated with Calyculin A and they woul...

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