Key features and details
- Rabbit polyclonal to HDAC5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HDAC5 antibody
See all HDAC5 primary antibodies
DescriptionRabbit polyclonal to HDAC5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human HDAC5 aa 500-550. The exact sequence is proprietary. (NP_005465.2).
Database link: Q9UQL6
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225906 was affinity purified using an epitope specific to HDAC5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 121 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
modificationsPhosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
- Information by UniProt
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
All lanes : Anti-HDAC5 antibody (ab225906) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Exposure time: 3 minutes
HDAC5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225906 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225906 at 0.4 µg/ml.
Lane 1: ab225906 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225906 has not yet been referenced specifically in any publications.