Overview

  • Product name

  • Description

    Rabbit polyclonal to HDAC5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human HDAC5 aa 600-650. The exact sequence is proprietary. NP_005465.2
    Database link: Q9UQL6

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Domain

      The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
    • Post-translational
      modifications

      Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
      Ubiquitinated. Polyubiquitination however does not lead to its degradation.
    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
    • Information by UniProt
    • Database links

    • Alternative names

      • Antigen NY CO 9 antibody
      • Antigen NY-CO-9 antibody
      • HD5 antibody
      • HDAC 5 antibody
      • HDAC5 antibody
      • HDAC5_HUMAN antibody
      • Histone deacetylase 5 antibody
      • NY CO 9 antibody
      see all

    Images

    • HDAC5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245409 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-HDAC5 antibody at 0.4 µg/ml.

      Lane 1: ab245409 IP in HeLa whole cell lysate.
      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab245409 has not yet been referenced specifically in any publications.

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