Anti-HDAC5 antibody (ab55403)
Key features and details
- Rabbit polyclonal to HDAC5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC5 antibody
See all HDAC5 primary antibodies -
Description
Rabbit polyclonal to HDAC5 -
Host species
Rabbit -
Specificity
ab55403 detects endogenous levels of total HDAC5 protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human HDAC5. Immunogen range: 225-274. Around Serine 259.
Database link: Q9UQL6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab55403 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
|
| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsPhosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK. - Information by UniProt
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Database links
- Entrez Gene: 10014 Human
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- Unigene: 438782 Human
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Alternative names
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 antibody (ab55403)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab55403 (1/50 dilution) in the presence and absence of the immunizing peptide.
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Western blot - Anti-HDAC5 antibody (ab55403)All lanes : Anti-HDAC5 antibody (ab55403) at 1/500 dilution
Lane 1 : HepG2 cell extract, without immunizing peptide
Lane 2 : HepG2 cell extract, with immunizing peptide
Predicted band size: 122 kDa
Observed band size: 122 kDa
Protocols
Datasheets and documents
References (9)
ab55403 has been referenced in 9 publications.
- Luo J et al. Epigenetic Regulation of IL-17-Induced Chemokines in Lung Epithelial Cells. Mediators Inflamm 2019:9050965 (2019). PubMed: 31080358
- Mazzocchi M et al. Gene Co-expression Analysis Identifies Histone Deacetylase 5 and 9 Expression in Midbrain Dopamine Neurons and as Regulators of Neurite Growth via Bone Morphogenetic Protein Signaling. Front Cell Dev Biol 7:191 (2019). PubMed: 31572723
- Yano M et al. Association of histone deacetylase expression with histology and prognosis of ovarian cancer. Oncol Lett 15:3524-3531 (2018). PubMed: 29456726
- Wanek J et al. Pharmacological Inhibition of Class IIA HDACs by LMK-235 in Pancreatic Neuroendocrine Tumor Cells. Int J Mol Sci 19:N/A (2018). PubMed: 30321986
- Jia HY et al. HDAC5 Inhibits Hepatic Lipogenic Genes Expression by Attenuating the Transcriptional Activity of Liver X Receptor. Cell Physiol Biochem 39:1561-7 (2016). PubMed: 27614433
- Nichols CB et al. ß-adrenergic signaling inhibits Gq-dependent protein kinase D activation by preventing protein kinase D translocation. Circ Res 114:1398-409 (2014). WB . PubMed: 24643961
- Takase K et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013). ICC/IF ; Mouse . PubMed: 23469282
- Funato H et al. Fasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus. PLoS One 6:e18950 (2011). IHC-FoFr ; Mouse . PubMed: 21526203
- Milde T et al. HDAC5 and HDAC9 in medulloblastoma: novel markers for risk stratification and role in tumor cell growth. Clin Cancer Res 16:3240-52 (2010). IHC-P ; Human . PubMed: 20413433
