Anti-HDAC5 antibody (ab55403)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameAnti-HDAC5 antibody
See all HDAC5 primary antibodies -
DescriptionRabbit polyclonal to HDAC5
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Host speciesRabbit
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Specificityab55403 detects endogenous levels of total HDAC5 protein.
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Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IF, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide corresponding to Human HDAC5. Immunogen range: 225-274. Around Serine 259.
Database link: Q9UQL6 -
Positive control
- Human breast carcinoma tissue and HepG2 cell extract.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab55403 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab55403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/5000. | |
| ICC/IF | 1/100. PubMed: 23469282 | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 21526203 |
Target
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FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
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DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
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Post-translational
modificationsPhosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation. -
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
- Information by UniProt
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Database links
- Entrez Gene: 10014 Human
- Entrez Gene: 15184 Mouse
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- SwissProt: Q9Z2V6 Mouse
- Unigene: 438782 Human
- Unigene: 22665 Mouse
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Alternative names
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 antibody (ab55403)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab55403 (1/50 dilution) in the presence and absence of the immunizing peptide. -
Western blot - Anti-HDAC5 antibody (ab55403)All lanes : Anti-HDAC5 antibody (ab55403) at 1/500 dilution
Lane 1 : HepG2 cell extract, without immunizing peptide
Lane 2 : HepG2 cell extract, with immunizing peptide
Predicted band size: 122 kDa
Observed band size: 122 kDa
Datasheets and documents
Protocols
References
This product has been referenced in:
- Yano M et al. Association of histone deacetylase expression with histology and prognosis of ovarian cancer. Oncol Lett 15:3524-3531 (2018). Read more (PubMed: 29456726) »
- Nichols CB et al. ß-adrenergic signaling inhibits Gq-dependent protein kinase D activation by preventing protein kinase D translocation. Circ Res 114:1398-409 (2014). WB . Read more (PubMed: 24643961) »
