Overview

  • Product name

  • Description

    Rabbit polyclonal to HDAC5
  • Host species

    Rabbit
  • Specificity

    ab55403 detects endogenous levels of total HDAC5 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ELISA, ICC/IF, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human HDAC5. Immunogen range: 225-274. Around Serine 259.
    Database link: Q9UQL6

  • Positive control

    • Human breast carcinoma tissue and HepG2 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab55403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
ICC/IF 1/100. PubMed: 23469282
IHC-FoFr Use at an assay dependent concentration. PubMed: 21526203

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain

    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications

    Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab55403 (1/50 dilution) in the presence and absence of the immunizing peptide.
  • All lanes : Anti-HDAC5 antibody (ab55403) at 1/500 dilution

    Lane 1 : HepG2 cell extract, without immunizing peptide
    Lane 2 : HepG2 cell extract, with immunizing peptide

    Predicted band size: 122 kDa
    Observed band size: 122 kDa

References

This product has been referenced in:

  • Yano M  et al. Association of histone deacetylase expression with histology and prognosis of ovarian cancer. Oncol Lett 15:3524-3531 (2018). Read more (PubMed: 29456726) »
  • Wanek J  et al. Pharmacological Inhibition of Class IIA HDACs by LMK-235 in Pancreatic Neuroendocrine Tumor Cells. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30321986) »
See all 6 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - BD permeabilization buffer
Sample
Mouse Cell (Mouse thymus tissue, thymocytes)
Specification
Mouse thymus tissue, thymocytes
Gating Strategy
CD45 positives
Preparation
Cell harvesting/tissue preparation method: Digestion with Collagenase D
Sample buffer: PBS 2% FCS

Mr. Sanjay Gawade

Verified customer

Submitted Jan 26 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain)
Loading amount
20 µg
Specification
Mouse brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 07 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (cardiomyocyte cell line)
Loading amount
10 µg
Specification
cardiomyocyte cell line
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Prof. Paul Townsend

Verified customer

Submitted Oct 18 2008

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