Product nameAnti-HDAC5 (phospho P498) antibody [EPR22996-105] - BSA and Azide free
See all HDAC5 primary antibodies
DescriptionRabbit monoclonal [EPR22996-105] to HDAC5 (phospho P498) - BSA and Azide free
Tested applicationsSuitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human HDAC5 aa 450-550 (phospho S498). The exact sequence is proprietary.
Database link: Q9UQL6
- WB: HeLa, NIH/3T3, and C2C12 whole cell lysates.
ab264561 is the carrier-free version of ab240644. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab264561 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab264561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 121 kDa.
|Dot blot||Use at an assay dependent concentration.
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
modificationsPhosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
- Information by UniProt
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
Dot blot analysis of HDAC5 (phospho S498) labeled with ab240644 at 1/1000 dilution.
Lane 1: HDAC5 (phospho S498) peptide (aa495-504).
Lane 2: HDAC5 non-phospho peptide (aa493-504).
Lane 3: HDAC4 (phospho S632) peptide (aa627-638).
Lane 4: HDAC7 (phospho S486) peptide (aa481-492).
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, Azide, Glycerol and BSA (ab240644).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264561 has not yet been referenced specifically in any publications.