Anti-HDAC5 (phospho S498) antibody (ab47283)
Key features and details
- Rabbit polyclonal to HDAC5 (phospho S498)
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-HDAC5 (phospho S498) antibody
See all HDAC5 primary antibodies -
Description
Rabbit polyclonal to HDAC5 (phospho S498) -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human HDAC5 (phospho S498). Immunogen range: 464-513
Database link: Q9UQL6 -
Positive control
- NIH/3T3 cell extracts; Breast carcinoma tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa).
|
IHC-P |
Use at an assay dependent concentration.
|
Notes |
---|
ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
-
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsPhosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK. - Information by UniProt
-
Database links
- Entrez Gene: 10014 Human
- Entrez Gene: 15184 Mouse
- Entrez Gene: 84580 Rat
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- SwissProt: Q9Z2V6 Mouse
- Unigene: 438782 Human
- Unigene: 22665 Mouse
-
Alternative names
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
see all
Images
-
All lanes : Anti-HDAC5 (phospho S498) antibody (ab47283) at 1/500 dilution
Lane 1 : Extracts from NIH/ 3T3 cells
Lane 2 : Extracts from NIH/ 3T3 cells; ab47283 preincubated with immunising peptide (blocking)
Predicted band size: 122 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted? -
ab47283 (1/100) staining HDAC5 (phospho S498) in paraffin embedded human breast carcinoma tissue. For the right hand panel, ab47283 was pre-incubated with the immunizing peptide (blocking).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (14)
ab47283 has been referenced in 14 publications.
- Truong V et al. Angiotensin II-induced histone deacetylase 5 phosphorylation, nuclear export, and Egr-1 expression are mediated by Akt pathway in A10 vascular smooth muscle cells. Am J Physiol Heart Circ Physiol 320:H1543-H1554 (2021). PubMed: 33606583
- Pietruczuk P et al. Protein kinase B mediates insulin-like growth factor 1-induced phosphorylation and nuclear export of histone deacetylase 5 via NADPH oxidase 4 activation in vascular smooth muscle cells. J Cell Physiol N/A:N/A (2019). PubMed: 30793765
- Tóth AD et al. Inflammation leads through PGE/EP3 signaling to HDAC5/MEF2-dependent transcription in cardiac myocytes. EMBO Mol Med 10:N/A (2018). PubMed: 29907596
- Meng ZX et al. Glucose Sensing by Skeletal Myocytes Couples Nutrient Signaling to Systemic Homeostasis. Mol Cell 66:332-344.e4 (2017). PubMed: 28475869
- Weeks KL et al. ß-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55a-PP2A-Mediated Dephosphorylation. J Am Heart Assoc 6:N/A (2017). PubMed: 28343149