Overview

  • Product name

    Anti-HDAC5 (phospho S498) antibody
    See all HDAC5 primary antibodies
  • Description

    Rabbit polyclonal to HDAC5 (phospho S498)
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for HDAC5, only when phosphorylated at Serine 498.
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HDAC5 (phospho S498). Immunogen range: 464-513
    Database link: Q9UQL6

  • Positive control

    • NIH/3T3 cell extracts; Breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab47283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain

    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications

    Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

Images

  • All lanes : Anti-HDAC5 (phospho S498) antibody (ab47283) at 1/500 dilution

    Lane 1 : Extracts from NIH/ 3T3 cells
    Lane 2 : Extracts from NIH/ 3T3 cells; ab47283 preincubated with immunising peptide (blocking)

    Predicted band size: 122 kDa
    Observed band size: 118 kDa
    why is the actual band size different from the predicted?

  • ab47283 (1/100) staining HDAC5 (phospho S498) in paraffin embedded human breast carcinoma tissue. For the right hand panel, ab47283 was pre-incubated with the immunizing peptide (blocking).

References

This product has been referenced in:

  • Pietruczuk P  et al. Protein kinase B mediates insulin-like growth factor 1-induced phosphorylation and nuclear export of histone deacetylase 5 via NADPH oxidase 4 activation in vascular smooth muscle cells. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30793765) »
  • Tóth AD  et al. Inflammation leads through PGE/EP3 signaling to HDAC5/MEF2-dependent transcription in cardiac myocytes. EMBO Mol Med 10:N/A (2018). Read more (PubMed: 29907596) »
See all 13 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (5%)
Sample
Rat Cell lysate - whole cell (Hippocampal primary Neurons)
Specification
Hippocampal primary Neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

Submitted Nov 24 2014

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