Anti-HDAC6 antibody [3D2] (ab253034)
![Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [3D2] (ab253034)](https://www.abcam.com/ps/products/253/ab253034/Images/ab253034-343573-Mouse-monoclonal-3D2-to-HDAC6-immunocytochemistry-hek293t-shsy5y-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [3D2] (ab253034)")
Key features and details
- Mouse monoclonal [3D2] to HDAC6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC6 antibody [3D2]
See all HDAC6 primary antibodies -
Description
Mouse monoclonal [3D2] to HDAC6 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human HDAC6.
Database link: Q9UBN7 -
Epitope
D2 domain of HDAC6 (region amino acids 471-481). -
Positive control
- ICC/IF: HEK-293T and SH-SY5Y cells. WB: HEK-293T lysate.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called A1487 Anti-HDAC6 Monoclonal Antibody (D2 domain-specific).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. -
Clonality
Monoclonal -
Clone number
3D2 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 131 kDa.
|
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| ICC/IF |
Use a concentration of 5 - 50 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 131 kDa. |
|
ICC/IF
Use a concentration of 5 - 50 µg/ml. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 10013 Human
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- Unigene: 6764 Human
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Alternative names
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [3D2] (ab253034)
Formaldehyde-fixed, Triton permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) (Panel A) and SH-SY5Y (Human neuroblastoma cell line from bone marrow) (Panel B) cells labeling HDAC6 using ab253034 at 25 µg/ml, overnight (green) followed by Alexa Fluor® 488 secondary antibody and Alexa Fluor® 647 secondary antibody respectively, in ICC/IF. Nuclear probe is visualized in blue (DAPI). Tubulin is visualized in Red.
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![Western blot - Anti-HDAC6 antibody [3D2] (ab253034)](https://www.abcam.com/ps/products/253/ab253034/Images/ab253034-343572-Mouse-monoclonal-3D2-to-HDAC6-western-blot-hek293t-human.jpg)
Western blot - Anti-HDAC6 antibody [3D2] (ab253034)Anti-HDAC6 antibody [3D2] (ab253034) at 1 µg/ml + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
HRP-conjugated anti-mouse IgG
Predicted band size: 131 kDaMembrane was blocked with 5% milk.
Reducing conditions using PBS/0.05% Tween-20 buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab253034 has not yet been referenced specifically in any publications.
