Overview

  • Product name
  • Description
    Rabbit polyclonal to HDAC6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human HDAC6 aa 7-56 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9UBN7

  • Positive control
    • Human intestine (ganglion cells) and Human testis tissues; HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab117516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.
ELISA 1/1000.
IHC-P 1/200.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization
    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Images

  • All lanes : Anti-HDAC6 antibody (ab117516) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with immunizing peptide

    Predicted band size: 131 kDa

  • ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human intestine tissue (ganglion cells) by Immunohistochemistry.

  • ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.

  • ab117516, at 1/100, staining HDAC6 in HepG2 cells by Immunofluorescence (Left image).
    The image on the right is treated with the synthesized peptide.

References

This product has been referenced in:
  • Fan T  et al. Autophagy Activation by Rapamycin Before Hypoxia-Reoxygenation Reduces Endoplasmic Reticulum Stress in Alveolar Epithelial Cells. Cell Physiol Biochem 41:79-90 (2017). Read more (PubMed: 28214844) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Sep 26 2017

Application
Western blot
Sample
Human Cell lysate - nuclear (LNCaP - Prostate Cancer Cells)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
35 µg
Specification
LNCaP - Prostate Cancer Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted May 09 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 07 2017

Answer

Thank you for your enquiry. I am sorry to confirm that as far as we are aware,  ab117516  Anti-HDAC6 antibody has not been tested and is therefore not guaranteed in mouse. I would be very pleased to hear to provide you with a discount code for the customer to test this antibody in mouse. DISCOUNT CODE: ##### Expiration date: ###### This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. We look forward to receiving your Abreview and wish you luck with your research.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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