Anti-HDAC6 antibody (ab117516)
Key features and details
- Rabbit polyclonal to HDAC6
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC6 antibody
See all HDAC6 primary antibodies -
Description
Rabbit polyclonal to HDAC6 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human HDAC6 aa 7-56 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9UBN7 -
Positive control
- Human intestine (ganglion cells) and Human testis tissues; HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+, Ca2+ -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 131 kDa.
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IHC-P |
1/200.
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ICC/IF | (4) |
1/100 - 1/500.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 131 kDa. |
IHC-P
1/200. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
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Alternative names
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
Images
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All lanes : Anti-HDAC6 antibody (ab117516) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with immunizing peptide
Predicted band size: 131 kDa -
ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human intestine tissue (ganglion cells) by Immunohistochemistry.
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ab117516, at 1:200 dilution, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.
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ab117516, at 1/100, staining HDAC6 in HepG2 cells by Immunofluorescence (Left image).
The image on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab117516 has been referenced in 3 publications.
- Manca S et al. The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer. Mol Cancer Res 17:225-237 (2019). PubMed: 30224543
- Fan T et al. Autophagy Activation by Rapamycin Before Hypoxia-Reoxygenation Reduces Endoplasmic Reticulum Stress in Alveolar Epithelial Cells. Cell Physiol Biochem 41:79-90 (2017). PubMed: 28214844
- Hanigan TW et al. Divergent JNK Phosphorylation of HDAC3 in Triple-Negative Breast Cancer Cells Determines HDAC Inhibitor Binding and Selectivity. Cell Chem Biol 24:1356-1367.e8 (2017). PubMed: 28943357