• Product name

  • Description

    Rabbit polyclonal to HDAC6
  • Host species

  • Specificity

    The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse) - N terminal.

  • Positive control

    • WB: Jurkat cell lysate.



Our Abpromise guarantee covers the use of ab1440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa). Block with 5% milk in TBST and incubate the antibody in blocking buffer.


  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational

    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization

    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all


  • Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate

    Predicted band size: 131 kDa
    Observed band size: 131 kDa


This product has been referenced in:

  • Xu Q  et al. Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells. Mol Cell Biochem 450:87-96 (2019). Read more (PubMed: 29846859) »
  • Yano M  et al. Clinicopathological correlation of ARID1A status with HDAC6 and its related factors in ovarian clear cell carcinoma. Sci Rep 9:2397 (2019). Read more (PubMed: 30787326) »
See all 22 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (jurkat)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Blocking step
tried both Milk and BSA individually as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 31 2018

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Mouse Cell (Neural cell)
Neural cell
Yes - 0.3% TritonX-100 in PBS for 30 minutes

Abcam user community

Verified customer

Submitted Oct 20 2014

Western blot
Rat Cell lysate - whole cell (Primary Neurons)
Loading amount
20 µg
Primary Neurons
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 15 2013


Thank you for your reply.

I looked up the order associated with ab1440, and it look as though you received that product for free as part of our 4for3 promotion a month ago. Since you received it for free, there is no cost to refund, but I am more than happy to send you a free of charge replacement antibody of your choice.

Please let me know if there is anything else I can help you with.

Read More


Thank you for providing that image.

Based on the information you have provided, I do beleive that there a re protocol varations that I can suggest that will help resolve this situation, therefore I would be happy to replace or refund the cost of the antibody.

Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody, that would be very helpful.

Read More


Thank you for contacting Abcam.

I am sorry that you are having some issues with ab1440. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

To able to try and aid you further in this matter, would you be able to provide an image of the results that you are recieving, when you use this antibody?

I look forward to your reply and helping to resolve this issue.

Read More


Thank you for your enquiry. I have been in touch with our lab team and they have informed me that for the application of this antibody by IHC using paraffin embedded sections antigen retrieval should be performed by heating sections in a microwave for 1 minute at high power before 10 minutes at medium power in 10 mM sodium citrate buffer. Keep slides fully immersed. For a positive control; human tonsil tissue sections can be used. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1440. This antibody has been tested with HeLa cell lysate (20 ug) and a ~134 kDa band was detected. The antibody should with mouse samples as well. Can you tell me specifically the type of mouse samples that you are using? Can you also send an image of your blot? Thank you and I look forward to hearing from you.

Read More


The immunogen used was a synthetic peptide mapping to Mouse HDAC-6 N-terminus. If you have any additional questions, please contact us again.

Read More


Thank you for the information. I have instructed our accounting department to issue you a refund for ab1440 and ab1437. This process does take a little while and if you have any questions regarding the refund, please contact our accounts department at accounts@abcam.com. If you have any additional questions, please let me know.

Read More

1-10 of 16 Abreviews or Q&A

For licensing inquiries, please contact partnerships@abcam.com

Sign up