Anti-HDAC6 antibody (ab1440)

Reviews (3)Q&A (13)References (26)
Western blot - Anti-HDAC6 antibody (ab1440)

    Key features and details

    • Rabbit polyclonal to HDAC6
    • Suitable for: IHC-P, WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-HDAC6 antibody
      See all HDAC6 primary antibodies

    • Description

      Rabbit polyclonal to HDAC6

    • Host species

      Rabbit

    • Specificity

      The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.

    • Tested applications

      Suitable for: IHC-P, WBmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Human

    • Immunogen

      Synthetic peptide (Mouse) - N terminal.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1440 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent concentration.
    WB (2)
    Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa). Block with 5% milk in TBST and incubate the antibody in blocking buffer.
    Notes
    IHC-P
    Use at an assay dependent concentration.
    WB
    Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa). Block with 5% milk in TBST and incubate the antibody in blocking buffer.

    Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
      Contains 1 UBP-type zinc finger.

    • Post-translational
      modifications

      Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
      Sumoylated in vitro.

    • Cellular localization

      Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
    • Information by UniProt

    • Database links

      • Alternative names

        • CPBHM antibody
        • FLJ16239 antibody
        • HD 6 antibody
        • HD6 antibody
        • HDAC 6 antibody
        • HDAC6 antibody
        • HDAC6_HUMAN antibody
        • Histone deacetylase 6 (HD6) antibody
        • Histone deacetylase 6 antibody
        • JM 21 antibody
        • JM21 antibody
        • KIAA0901 antibody
        • OTTHUMP00000032398 antibody
        • OTTHUMP00000197663 antibody
        • PPP1R90 antibody
        • Protein phosphatase 1 regulatory subunit 90 antibody
        see all

      Images

      • Western blot - Anti-HDAC6 antibody (ab1440)
        Western blot - Anti-HDAC6 antibody (ab1440)
        Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate

        Predicted band size: 131 kDa
        Observed band size: 131 kDa

      References (26)

      Publishing research using ab1440? Please let us know so that we can cite the reference in this datasheet.

      ab1440 has been referenced in 26 publications.

      • Guo SD  et al. HDAC6 promotes sepsis development by impairing PHB1-mediated mitochondrial respiratory chain function. Aging (Albany NY) 12:5411-5422 (2020). PubMed: 32221047
      • Wang N  et al. ACY-1215, a HDAC6 inhibitor, decreases the dexamethasone-induced suppression of osteogenesis in MC3T3-E1 cells. Mol Med Rep 22:2451-2459 (2020). PubMed: 32705192
      • Silva JM  et al. Dysregulation of autophagy and stress granule-related proteins in stress-driven Tau pathology. Cell Death Differ 26:1411-1427 (2019). PubMed: 30442948
      • Schlüter A  et al. Histone Deacetylases Contribute to Excitotoxicity-Triggered Degeneration of Retinal Ganglion Cells In Vivo. Mol Neurobiol 56:8018-8034 (2019). PubMed: 31161423
      • Xu Q  et al. Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells. Mol Cell Biochem 450:87-96 (2019). PubMed: 29846859
      View all Publications for this product

      Customer reviews and Q&As

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      1-10 of 16 Abreviews or Q&A

      Western blot abreview for Anti-HDAC6 antibody

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (jurkat)
      Gel Running Conditions
      Reduced Denaturing (8)
      Loading amount
      30 µg
      Specification
      jurkat
      Blocking step
      tried both Milk and BSA individually as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 31 2018

      Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC6 antibody

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
      Sample
      Mouse Cell (Neural cell)
      Specification
      Neural cell
      Permeabilization
      Yes - 0.3% TritonX-100 in PBS for 30 minutes
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 20 2014

      Western blot abreview for Anti-HDAC6 antibody

       Inconclusive
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (Primary Neurons)
      Loading amount
      20 µg
      Specification
      Primary Neurons
      Gel Running Conditions
      Reduced Denaturing (4-12)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 15 2013

      Question



      We would like to proceed with a refund. The PO# is *. Please let me know if any other information is needed.

      Read More
      Answer

      Thank you for your reply.

      I looked up the order associated with ab1440, and it look as though you received that product for free as part of our 4for3 promotion a month ago. Since you received it for free, there is no cost to refund, but I am more than happy to send you a free of charge replacement antibody of your choice.

      Please let me know if there is anything else I can help you with.

      Read More

      Question

      Please find the western blots for HDAC6 at three different exposure times (flash, 15 seconds, and 5 minutes).

      Read More
      Answer

      Thank you for providing that image.

      Based on the information you have provided, I do beleive that there a re protocol varations that I can suggest that will help resolve this situation, therefore I would be happy to replace or refund the cost of the antibody.

      Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody, that would be very helpful.

      Read More

      Question

      Amount of protein loaded: 20 mcg Blocking conditions: 1 hour in 5% NFMP TBST Detection method: Femto ECL and film Electrophoresis and transfer conditions: 12.5% acrylamide gel, 60 minute 15 V semidry transfer Primary antibody conditions (diluent/dilution, incubation time/temperature): 1 mcg/mL in 2.5% NFMP TBST at 4C overnight Secondary antibody conditions (diluent/dilution, incubation time/temperature): 1:2000 goat anti rabbit HRP in 2.5% NFMP TBST 1 hour at room temperature Wash steps: 5 minutes in TBST 3 times, 5 minutes in TBS 2 times prior to developing Primary band is detected around 80-90 kDa instead of the expected 131 kDa. Further development reveals significant secondary bands. Protein was isolated from 5637 human bladder cancer cell line lysed in RIPA with protease inhibitors.

      Read More
      Answer

      Thank you for contacting Abcam.

      I am sorry that you are having some issues with ab1440. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

      To able to try and aid you further in this matter, would you be able to provide an image of the results that you are recieving, when you use this antibody?

      I look forward to your reply and helping to resolve this issue.

      Read More

      Question

      I need more detailed information about the immunohistochemical procedure concerning yuor product Anti-HDAC6 ab1440 . I'm going to use ab1440 on formalin-fixed, paraffin-embedded rat tissues and I want to know the exact antigen retrival procedure requested (if necessary). Could you also suggest me a particular rat tissue that constitutively shows prominent HDAC6-IHC expression (positive control)? Thank you for your precious information Best Regards

      Read More
      Answer

      Thank you for your enquiry. I have been in touch with our lab team and they have informed me that for the application of this antibody by IHC using paraffin embedded sections antigen retrieval should be performed by heating sections in a microwave for 1 minute at high power before 10 minutes at medium power in 10 mM sodium citrate buffer. Keep slides fully immersed. For a positive control; human tonsil tissue sections can be used. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      BATCH NUMBER 163987 ORDER NUMBER 122931 DESCRIPTION OF THE PROBLEM The size of HDAC 6 should be around 134 kDA, but instead, I get 80 kDA each time SAMPLE mouse,whole cell extract PRIMARY ANTIBODY 1:1000 DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED we re-blot the same membrane with [competitor's]HDAc6 Ab and got correct size band ANTIBODY STORAGE CONDITIONS -20 SAMPLE PREPARATION standard buffer with protease inhibitors AMOUNT OF PROTEIN LOADED 100ug ELECTROPHORESIS/GEL CONDITIONS 6% reducing gel TRANSFER AND BLOCKING CONDITIONS standard SECONDARY ANTIBODY standard protocol HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? no

      Read More
      Answer

      Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1440. This antibody has been tested with HeLa cell lysate (20 ug) and a ~134 kDa band was detected. The antibody should with mouse samples as well. Can you tell me specifically the type of mouse samples that you are using? Can you also send an image of your blot? Thank you and I look forward to hearing from you.

      Read More

      Question

      Could you tell me the immunogen for this antibody? I check the other 2 Anti-HDAC6 antibody you have. They all provide the epitope used to generated the antibody, but this one not.

      Read More
      Answer

      The immunogen used was a synthetic peptide mapping to Mouse HDAC-6 N-terminus. If you have any additional questions, please contact us again.

      Read More

      Question

      I have had the exact same problem with HDAC antibodies 4 and 6. I have failed to detect them on a western using Hela extract. The blots were completely blank. I went upto 20ug of Hela extract. As a control, I blotted with HDAC 1 antibody. HDAC 1 gives a nice band of the right size; HDAC4 and 6 lanes are blank. Experiment was repeated 2 times. Got the same result.

      Read More
      Answer

      Thank you for the information. I have instructed our accounting department to issue you a refund for ab1440 and ab1437. This process does take a little while and if you have any questions regarding the refund, please contact our accounts department at accounts@abcam.com. If you have any additional questions, please let me know.

      Read More

      1-10 of 16 Abreviews or Q&A

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