Anti-HDAC6 antibody (ab1440)

Reviews (3)Q&A (13)References (17)

Overview

  • Product name
    Anti-HDAC6 antibody
    See all HDAC6 primary antibodies
  • Description
    Rabbit polyclonal to HDAC6
  • Host species
    Rabbit
  • Specificity
    The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse) - N terminal.

  • Positive control
    • WB: Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab1440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa).Can be blocked with HDAC6 peptide (ab41708). Block with 5% milk in TBST and incubate the antibody in blocking buffer.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization
    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links
    • Alternative names
      • CPBHM antibody
      • FLJ16239 antibody
      • HD 6 antibody
      • HD6 antibody
      • HDAC 6 antibody
      • HDAC6 antibody
      • HDAC6_HUMAN antibody
      • Histone deacetylase 6 (HD6) antibody
      • Histone deacetylase 6 antibody
      • JM 21 antibody
      • JM21 antibody
      • KIAA0901 antibody
      • OTTHUMP00000032398 antibody
      • OTTHUMP00000197663 antibody
      • PPP1R90 antibody
      • Protein phosphatase 1 regulatory subunit 90 antibody
      see all

    Images

    • Western blot - Anti-HDAC6 antibody (ab1440)
      Western blot - Anti-HDAC6 antibody (ab1440)
      Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate

      Predicted band size: 131 kDa
      Observed band size: 131 kDa

    References

    This product has been referenced in:
    • Yano M  et al. Association of histone deacetylase expression with histology and prognosis of ovarian cancer. Oncol Lett 15:3524-3531 (2018). Read more (PubMed: 29456726) »
    • Li C  et al. The immunohistochemical expression and potential prognostic value of HDAC6 and AR in invasive breast cancer. Hum Pathol 75:16-25 (2018). Read more (PubMed: 29180246) »
    See all 17 Publications for this product

    Publishing research using ab1440? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-10 of 16 Abreviews or Q&A

    Western blot abreview for Anti-HDAC6 antibody

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (jurkat)
    Gel Running Conditions
    Reduced Denaturing (8)
    Loading amount
    30 µg
    Specification
    jurkat
    Blocking step
    tried both Milk and BSA individually as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 31 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC6 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
    Sample
    Mouse Cell (Neural cell)
    Specification
    Neural cell
    Permeabilization
    Yes - 0.3% TritonX-100 in PBS for 30 minutes
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 20 2014

    Western blot abreview for Anti-HDAC6 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Primary Neurons)
    Loading amount
    20 µg
    Specification
    Primary Neurons
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 15 2013

    Question



    We would like to proceed with a refund. The PO# is *. Please let me know if any other information is needed.

    Read More
    Answer

    Thank you for your reply.

    I looked up the order associated with ab1440, and it look as though you received that product for free as part of our 4for3 promotion a month ago. Since you received it for free, there is no cost to refund, but I am more than happy to send you a free of charge replacement antibody of your choice.

    Please let me know if there is anything else I can help you with.

    Read More

    Question

    Please find the western blots for HDAC6 at three different exposure times (flash, 15 seconds, and 5 minutes).

    Read More
    Answer

    Thank you for providing that image.

    Based on the information you have provided, I do beleive that there a re protocol varations that I can suggest that will help resolve this situation, therefore I would be happy to replace or refund the cost of the antibody.

    Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody, that would be very helpful.

    Read More

    Question

    Amount of protein loaded: 20 mcg Blocking conditions: 1 hour in 5% NFMP TBST Detection method: Femto ECL and film Electrophoresis and transfer conditions: 12.5% acrylamide gel, 60 minute 15 V semidry transfer Primary antibody conditions (diluent/dilution, incubation time/temperature): 1 mcg/mL in 2.5% NFMP TBST at 4C overnight Secondary antibody conditions (diluent/dilution, incubation time/temperature): 1:2000 goat anti rabbit HRP in 2.5% NFMP TBST 1 hour at room temperature Wash steps: 5 minutes in TBST 3 times, 5 minutes in TBS 2 times prior to developing Primary band is detected around 80-90 kDa instead of the expected 131 kDa. Further development reveals significant secondary bands. Protein was isolated from 5637 human bladder cancer cell line lysed in RIPA with protease inhibitors.

    Read More
    Answer

    Thank you for contacting Abcam.

    I am sorry that you are having some issues with ab1440. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

    To able to try and aid you further in this matter, would you be able to provide an image of the results that you are recieving, when you use this antibody?

    I look forward to your reply and helping to resolve this issue.

    Read More

    Question

    I need more detailed information about the immunohistochemical procedure concerning yuor product Anti-HDAC6 ab1440 . I'm going to use ab1440 on formalin-fixed, paraffin-embedded rat tissues and I want to know the exact antigen retrival procedure requested (if necessary). Could you also suggest me a particular rat tissue that constitutively shows prominent HDAC6-IHC expression (positive control)? Thank you for your precious information Best Regards

    Read More
    Answer

    Thank you for your enquiry. I have been in touch with our lab team and they have informed me that for the application of this antibody by IHC using paraffin embedded sections antigen retrieval should be performed by heating sections in a microwave for 1 minute at high power before 10 minutes at medium power in 10 mM sodium citrate buffer. Keep slides fully immersed. For a positive control; human tonsil tissue sections can be used. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    BATCH NUMBER 163987 ORDER NUMBER 122931 DESCRIPTION OF THE PROBLEM The size of HDAC 6 should be around 134 kDA, but instead, I get 80 kDA each time SAMPLE mouse,whole cell extract PRIMARY ANTIBODY 1:1000 DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED we re-blot the same membrane with [competitor's]HDAc6 Ab and got correct size band ANTIBODY STORAGE CONDITIONS -20 SAMPLE PREPARATION standard buffer with protease inhibitors AMOUNT OF PROTEIN LOADED 100ug ELECTROPHORESIS/GEL CONDITIONS 6% reducing gel TRANSFER AND BLOCKING CONDITIONS standard SECONDARY ANTIBODY standard protocol HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? no

    Read More
    Answer

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1440. This antibody has been tested with HeLa cell lysate (20 ug) and a ~134 kDa band was detected. The antibody should with mouse samples as well. Can you tell me specifically the type of mouse samples that you are using? Can you also send an image of your blot? Thank you and I look forward to hearing from you.

    Read More

    Question

    Could you tell me the immunogen for this antibody? I check the other 2 Anti-HDAC6 antibody you have. They all provide the epitope used to generated the antibody, but this one not.

    Read More
    Answer

    The immunogen used was a synthetic peptide mapping to Mouse HDAC-6 N-terminus. If you have any additional questions, please contact us again.

    Read More

    Question

    I have had the exact same problem with HDAC antibodies 4 and 6. I have failed to detect them on a western using Hela extract. The blots were completely blank. I went upto 20ug of Hela extract. As a control, I blotted with HDAC 1 antibody. HDAC 1 gives a nice band of the right size; HDAC4 and 6 lanes are blank. Experiment was repeated 2 times. Got the same result.

    Read More
    Answer

    Thank you for the information. I have instructed our accounting department to issue you a refund for ab1440 and ab1437. This process does take a little while and if you have any questions regarding the refund, please contact our accounts department at accounts@abcam.com. If you have any additional questions, please let me know.

    Read More

    1-10 of 16 Abreviews or Q&A

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