Overview

  • Product name

  • Description

    Rabbit polyclonal to HDAC6
  • Host species

    Rabbit
  • Specificity

    The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse) - N terminal.

  • Positive control

    • WB: Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab1440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa). Block with 5% milk in TBST and incubate the antibody in blocking buffer.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications

    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization

    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Images

  • Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate

    Predicted band size: 131 kDa
    Observed band size: 131 kDa

References

This product has been referenced in:

  • Xu Q  et al. Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells. Mol Cell Biochem 450:87-96 (2019). Read more (PubMed: 29846859) »
  • Yano M  et al. Clinicopathological correlation of ARID1A status with HDAC6 and its related factors in ovarian clear cell carcinoma. Sci Rep 9:2397 (2019). Read more (PubMed: 30787326) »
See all 22 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (jurkat)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Specification
jurkat
Blocking step
tried both Milk and BSA individually as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 31 2018

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Sample
Mouse Cell (Neural cell)
Specification
Neural cell
Permeabilization
Yes - 0.3% TritonX-100 in PBS for 30 minutes
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 20 2014

Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Loading amount
20 µg
Specification
Primary Neurons
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 15 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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