The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa).Can be blocked with HDAC6 peptide (ab41708). Block with 5% milk in TBST and incubate the antibody in blocking buffer.
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger.
Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.