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BATCH NUMBER 163987 ORDER NUMBER 122931 DESCRIPTION OF THE PROBLEM The size of HDAC 6 should be around 134 kDA, but instead, I get 80 kDA each time SAMPLE mouse,whole cell extract PRIMARY ANTIBODY 1:1000 DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED we re-blot the same membrane with [competitor's]HDAc6 Ab and got correct size band ANTIBODY STORAGE CONDITIONS -20 SAMPLE PREPARATION standard buffer with protease inhibitors AMOUNT OF PROTEIN LOADED 100ug ELECTROPHORESIS/GEL CONDITIONS 6% reducing gel TRANSFER AND BLOCKING CONDITIONS standard SECONDARY ANTIBODY standard protocol HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? no
Asked on Feb 16 2006
Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1440. This antibody has been tested with HeLa cell lysate (20 ug) and a ~134 kDa band was detected. The antibody should with mouse samples as well. Can you tell me specifically the type of mouse samples that you are using? Can you also send an image of your blot? Thank you and I look forward to hearing from you.
Answered on Feb 20 2006