Western blot abreview for Anti-HDAC6 antibody

Inconclusive
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Loading amount
20 µg
Specification
Primary Neurons
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/100
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TPBS 0.05%

Secondary antibody

Name
Non-Abcam antibody was used: anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: ~130 kDa Non-specific: ~300, ~250, ~160, ~1 kDa
Positive control
sample

Additional data

Abcam response
Thank you for submitting an Abreview of ab1440. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
Additionally, this antibody is covered by our Abpromise, so if the antibody was purchased within the past 12 months, I would be happy to discuss a refund or replacement with you to resolve this issue.
I look forward to your reply.

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Verified customer

Submitted May 15 2013

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