Key features and details
- Rabbit polyclonal to HDAC6
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-HDAC6 antibody
See all HDAC6 primary antibodies
DescriptionRabbit polyclonal to HDAC6
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment (His-tag) corresponding to Human HDAC6 aa 166-348. N-terminal tag. (Expressed in E.coli).
VLADTYDSVYLHPNSYSCACLASGSVLRLVDAVLGAEIRNGMAIIRPPGH HAQHSLMDGYCMFNHVAVAARYAQQKHRIRRVLIVDWDVHHGQGTQFTFD QDPSVLYFSIHRYEQGRFWPHLKASNWSTTGFGQGQGYTINVPWNQVGMR DADYIAAFLHVLLPVALEFQPQLVLVAAGFDAL
Database link: Q9UBN7-1
- WB: Pig kidney lysate; Recombinant human HDAC6 protein. IHC-P: Human kidney, liver and liver cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localizationNucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
- Information by UniProt
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for HDAC6 using ab233520 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for HDAC6 using ab233520 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for HDAC6 using ab233520 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Anti-HDAC6 antibody (ab233520) at 2 µg/ml + Pig kidney lysate
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Anti-HDAC6 antibody (ab233520) at 2 µg/ml + Recombinant human HDAC6 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233520 has not yet been referenced specifically in any publications.