Overview

  • Product name
    Anti-HDAC6 antibody
    See all HDAC6 primary antibodies
  • Description
    Rabbit polyclonal to HDAC6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human HDAC6.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab93301.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; HEK293; HUVEC.

Properties

Applications

Our Abpromise guarantee covers the use of ab82557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa).

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization
    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links
    • Alternative names
      • CPBHM antibody
      • FLJ16239 antibody
      • HD 6 antibody
      • HD6 antibody
      • HDAC 6 antibody
      • HDAC6 antibody
      • HDAC6_HUMAN antibody
      • Histone deacetylase 6 (HD6) antibody
      • Histone deacetylase 6 antibody
      • JM 21 antibody
      • JM21 antibody
      • KIAA0901 antibody
      • OTTHUMP00000032398 antibody
      • OTTHUMP00000197663 antibody
      • PPP1R90 antibody
      • Protein phosphatase 1 regulatory subunit 90 antibody
      see all

    Images

    • Western blot - Anti-HDAC6 antibody (ab82557)
      Western blot - Anti-HDAC6 antibody (ab82557)
      All lanes : Anti-HDAC6 antibody (ab82557) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 131 kDa
      Observed band size: 150 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 18 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 15 minutes
    • Western blot - Anti-HDAC6 antibody (ab82557)
      Western blot - Anti-HDAC6 antibody (ab82557)
      Anti-HDAC6 antibody (ab82557) at 1 µg/ml + Rat primary neurons whole cell lysate at 20 µg

      Secondary
      Goat anti-rabbit IgG at 1/2000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 131 kDa
      Observed band size: 140 kDa (why is the actual band size different from the predicted?)


      Exposure time: 30 seconds


      Blocked with 5% milk for 1 hour at 23°C; followed by incubation with ab82557 for 15 hours in 5% BSA in TPBS 0.05% at 4°C.

      See Abreview

    References

    This product has been referenced in:
    • Guedes-Dias P  et al. HDAC6 inhibition induces mitochondrial fusion, autophagic flux and reduces diffuse mutant huntingtin in striatal neurons. Biochim Biophys Acta 1852:2484-93 (2015). Read more (PubMed: 26300485) »

    See 1 Publication for this product

    Publishing research using ab82557? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-HDAC6 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Rat Cell lysate - whole cell (Primary Neurons)
    Specification
    Primary Neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    Verified customer

    Submitted Jul 12 2013

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