Anti-HDAC6 antibody (ab82557)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-HDAC6 antibody
See all HDAC6 primary antibodies -
DescriptionRabbit polyclonal to HDAC6
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human HDAC6.
Read Abcam's proprietary immunogen policy (Peptide available as ab93301.) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; HEK293; HUVEC.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab82557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa). |
Target
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FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
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Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
Cellular localizationNucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
- Information by UniProt
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Database links
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Entrez Gene: 84581 Rat
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
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Alternative names
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
Images
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Western blot - Anti-HDAC6 antibody (ab82557)All lanes : Anti-HDAC6 antibody (ab82557) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes -
Western blot - Anti-HDAC6 antibody (ab82557)Anti-HDAC6 antibody (ab82557) at 1 µg/ml + Rat primary neurons whole cell lysate at 20 µg
Secondary
Goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocked with 5% milk for 1 hour at 23°C; followed by incubation with ab82557 for 15 hours in 5% BSA in TPBS 0.05% at 4°C.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Guedes-Dias P et al. HDAC6 inhibition induces mitochondrial fusion, autophagic flux and reduces diffuse mutant huntingtin in striatal neurons. Biochim Biophys Acta 1852:2484-93 (2015). Read more (PubMed: 26300485) »
